An immune-induced Reeler protein is involved in the Bombyx mori melanization cascade
An immune-induced Reeler protein is involved in the Bombyx mori melanization cascade
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免疫诱导的 Reeler 蛋白参与家蚕黑化级联
DOI：
10.1016/j.ibmb.2011.05.001
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发表时间：
2011-09-01
影响因子：
3.8
通讯作者：
Zhang, Chuan-Xi
中科院分区：
文献类型：
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作者：
Bao, Yan-Yuan;Xue, Jian;Zhang, Chuan-Xi
在本研究中，我们从受细菌攻击的家蚕幼虫脂肪体中分离到两个卷轴cDNAs。这两个基因的大部分编码区都有一个卷曲结构域，它们在家蚕幼虫组织中的表达模式不同。大肠杆菌K12和枯草芽孢杆菌对家蚕幼虫血细胞、脂肪体和中肠组织中的reeler1基因有较强的诱导作用，但在这些组织中的表达水平极低。我们将重点放在reeler1基因上进行功能分析。体内双链reeler1 RNA的干扰导致注射细菌的幼虫结节减少，而注射重组的reeler1促进了reeler1基因沉默幼虫的结节形成，表明Reeler1参与了结瘤反应。在细菌攻击的幼虫血淋巴中，reeler1基因的敲除显著降低了酚氧化酶的活性，而注射重组的reeler1则增强了酚氧化酶的活性，这表明reeler1参与了酚氧化酶原激活级联反应。我们的结果为昆虫免疫中的黑化级联提供了新的机制证据。(C)2011爱思唯尔有限公司。保留所有权利。
In this study, we isolated two reeler cDNAs from bacteria-challenged larval fat bodies of the silkworm, Bombyx mori. A reeler domain spanned most of the coding regions of these two cDNAs, and their expression patterns were different in B. mori larval tissues. The reeler1 gene was strongly induced by Escherichia coli K12 and Bacillus subtilis in B. mori larval hemocytes, fat bodies and midguts, but reeler2 was expressed at extremely low levels in these tissues. We focused on the reeler1 gene for functional analysis. Interference by double-stranded reeler1 RNA in vivo led to reduced nodule formation in bacteria-injected larvae, while the injection of recombinant Reeler1 promoted nodule formation in reeler1 gene-silenced larvae, indicating that Reeler1 is involved in the nodulation response. Knockdown of the reeler1 gene significantly decreased phenoloxidase activity in bacteria-challenged larval hemolymph, while injection of recombinant Reeler1 enhanced phenoloxidase activity, suggesting that Reeler1 is involved in the prophenoloxidase activation cascade. Our results provide new mechanistic evidence about the melanization cascade in the insect immunity. (C) 2011 Elsevier Ltd. All rights reserved.