Structure of HCVIRES domain II determined by NMR
Structure of HCVIRES domain II determined by NMR
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DOI：
10.1038/nsb1004
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发表时间：
2003-12-01
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影响因子：
--
通讯作者：
Puglisi, JD
中科院分区：
文献类型：
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作者：
Lukavsky, PJ;Kim, I;Puglisi, JD
复杂的RNA结构调节许多生物过程，但通常太大而无法通过NMR方法进行结构测定。丙型肝炎病毒（HCV）RNA基因组的5个非翻译区（5 UTR）含有一个内部核糖体进入位点（IRES），该位点以高亲和力和特异性结合40 S核糖体亚基以控制翻译。HCV IRES的结构域II形成可改变核糖体构象的25-kDa折叠亚结构域。我们在这里报告的结构域II确定使用NMR方法，结合短期和长期的结构数据。结构域II采用扭曲的L形结构，其游离形式的整体形状与其40 S亚基结合形式明显相似;这表明结构域II如何调节40 S亚基构象。结果表明NMR可用于大型生物RNA的结构分析。
Complex RNA structures regulate many biological processes, but are often too large for structure determination by NMR methods. The 5 untranslated region (5 UTR) of the hepatitis C viral (HCV) RNA genome contains an internal ribosome entry site (IRES) that binds to 40S ribosomal subunits with high affinity and specificity to control translation. Domain II of the HCV IRES forms a 25-kDa folded subdomain that may alter ribosome conformation. We report here the structure of domain II as determined using an NMR approach that combines short- and long-range structural data. Domain II adopts a distorted L-shape structure, and its overall shape in the free form is markedly similar to its 40S subunit bound form; this suggests how domain II may modulate 40S subunit conformation. The results show how NMR can be used for structural analysis of large biological RNAs.