RESISTANCE TO MAREKS-DISEASE IN CHICKENS WITH RECOMBINANT HAPLOTYPES OF THE MAJOR HISTOCOMPATIBILITY-(B) COMPLEX
RESISTANCE TO MAREKS-DISEASE IN CHICKENS WITH RECOMBINANT HAPLOTYPES OF THE MAJOR HISTOCOMPATIBILITY-(B) COMPLEX
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DOI：
10.3382/ps.0730502
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发表时间：
1994-04-01
期刊：
影响因子：
4.4
通讯作者：
BRILES, WE
中科院分区：
文献类型：
--
作者：
SCHAT, KA;TAYLOR, RL;BRILES, WE
对马立克氏病（MD）的遗传抗性与MHC的B-F区域相关。检测了具有两种MHC单倍型之一的鸡对不同MDV毒株攻击的抗性。将具有血清学相似MHC重组体B（R2）和B（R4）（均为B（F2-G23））的鸡与高度近交的UCD-003系（B17 B17）回交四代。杂合子（B17 BF 2-G23）交配产生BR 2BR 2和BR 4 BR 4纯合子与93%的背景基因的一致性。两种基因型均对GA-5 MDV具有高度抗性，BR 2BR 2和BR 4 BR 4的MD发病率分别为0和8%，而UCD-003鸡的MD发病率高于80%。用极强毒RB-LB毒株攻毒，在BR 2BR 2和BR 4 BR 4鸡中分别导致10%和31% MD，而在B17 B17（UCD-003）和B23 B23（新罕布什尔州105）系中分别导致100%和52% MD。在感染后5和6 d，BR 2BR 2和B23 B23基因型的病毒血症水平显著低于B17 B17基因型。
Genetic resistance to Marek's disease (MD) is associated with the B-F region of the MHC. The resistance of chickens possessing either of two MHC haplotypes to challenge with different strains of MDV was examined. Chickens with serologically similar MHC recombinants B(R2) and B(R4) (both B(F2-G23)) were backcrossed for four generations to the highly inbred UCD-003 line (B17B17). Heterozygotes (B17BF2-G23) were mated to produce BR2BR2 and BR4BR4 homozygotes with 93% background gene uniformity. Both genotypes were highly resistant to GA-5 MDV, having an incidence of 0 and 8% MD for BR2BR2 and BR4BR4, respectively, whereas the incidence of MD in the UCD-003 birds was above 80%. Challenge with the very virulent RB-LB strain caused 10% and 31% MD in the BR2BR2 and BR4BR4 chickens, respectively, compared with 100% and 52% in the B17B17 (UCD-003) and B23B23 (New Hampshire 105) lines, respectively. Viremia levels at 5 and 6 d postinfection were significantly lower in BR2BR2 and B23B23 than in B17B17 genotypes.