Single-Cell Delineation of Who's on First and Second Heart Fields During Development.
Single-Cell Delineation of Who's on First and Second Heart Fields During Development.
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DOI：
10.1161/circresaha.119.315576
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发表时间：
2019-08
影响因子：
20.1
通讯作者：
Francisco X. Galdos;Sean M. Wu
中科院分区：
文献类型：
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作者：
Francisco X. Galdos;Sean M. Wu
传统的谱系追踪和基因敲除研究为我们理解四腔心脏的发育起源提供了重要的见解。然而，导致心脏祖细胞早期分离的分子机制仍不清楚。在早期发育过程中，原肠胚中胚层产生两个暂时分离的祖细胞波，最终成为第一和第二心脏领域。第一心野（FHF）细胞的后代在左心室和左心房和右心房的一部分中发现，而第二心野（SHF）的后代在右心室、两个心房和流出道（OFT）中产生细胞。1虽然对将FHF与SHF细胞分离的标记物了解很多，指导这两个谱系分离的精确转录和信号线索仍然未知。然而，随着单细胞RNA测序（scRNA-seq）技术的出现，新的数据已经可以破译调节心脏发育和细胞身份的精确机制。
Traditional lineage tracing and gene knockout studies have provided significant insights into our understandings of the developmental origins of the 4 chambered heart. Yet, the molecular mechanisms that lead to the early segregation of cardiac progenitors remain unclear. During early development, gastrulating mesoderm gives rise to 2 temporally separated waves of progenitors that eventually become the first and second heart fields. Descendants of the first heart field (FHF) cells are found in the left ventricle and a portion of left and right atrium, while descendants of the second heart field (SHF) give rise to cells in right ventricle, both atria, and the outflow tract (OFT).1 While much is known about markers that segregate FHF from SHF cells, the precise transcriptional and signaling cues that direct the segregation of these 2 lineages remain unknown. With the advent of single-cell RNA-sequencing (scRNA-seq) technologies, however, new data have become available to decipher the precise mechanisms that regulate cardiac development and cellular identity.