Hyaline cartilage differentiation of fibroblasts in regeneration and regenerative medicine.
Hyaline cartilage differentiation of fibroblasts in regeneration and regenerative medicine.
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DOI：
10.1242/dev.200249
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发表时间：
2022-01-15
期刊：
影响因子：
--
通讯作者：
Muneoka K
中科院分区：
文献类型：
--
作者：
Yu L;Lin YL;Yan M;Li T;Wu EY;Zimmel K;Qureshi O;Falck A;Sherman KM;Huggins SS;Hurtado DO;Suva LJ;Gaddy D;Cai J;Brunauer R;Dawson LA;Muneoka K
哺乳动物的截肢损伤通常是不可再生的；然而，BMP9 治疗可刺激关节再生，表明存在潜在的关节软骨细胞祖细胞。 BMP9 在体内诱导一系列软骨形成基因，并且在截肢伤口细胞培养物中观察到类似的反应。 BMP9 处理细胞的扩展培养导致透明软骨分化，单细胞 RNAseq 分析确定伤口成纤维细胞对 BMP9 敏感。该培养模型用于鉴定 BMP9 反应性成体成纤维细胞系，并开发了一种培养策略来改造透明软骨，以便植入严重受损的关节中。移植的透明软骨在植入后存活并保持透明软骨表型，但没有形成成熟的关节软骨。此外，在一些样本中发现了个体肥大软骨细胞，表明急性关节损伤部位可以促进移植透明软骨的成骨进展。研究结果确定成纤维细胞是工程关节软骨的细胞来源，并建立了一种新的实验策略，弥合了再生生物学和再生医学之间的差距。摘要：体内关节软骨再生可作为开发软骨工程修复受损关节的新方法的模型，并将成纤维细胞鉴定为 BMP9 诱导型软骨祖细胞。
Amputation injuries in mammals are typically non-regenerative; however, joint regeneration is stimulated by BMP9 treatment, indicating the presence of latent articular chondrocyte progenitor cells. BMP9 induces a battery of chondrogenic genes in vivo, and a similar response is observed in cultures of amputation wound cells. Extended cultures of BMP9-treated cells results in differentiation of hyaline cartilage, and single cell RNAseq analysis identified wound fibroblasts as BMP9 responsive. This culture model was used to identify a BMP9-responsive adult fibroblast cell line and a culture strategy was developed to engineer hyaline cartilage for engraftment into an acutely damaged joint. Transplanted hyaline cartilage survived engraftment and maintained a hyaline cartilage phenotype, but did not form mature articular cartilage. In addition, individual hypertrophic chondrocytes were identified in some samples, indicating that the acute joint injury site can promote osteogenic progression of engrafted hyaline cartilage. The findings identify fibroblasts as a cell source for engineering articular cartilage and establish a novel experimental strategy that bridges the gap between regeneration biology and regenerative medicine. Summary: In vivo articular cartilage regeneration serves as a model to develop novel approaches for engineering cartilage to repair damaged joints and identifies fibroblasts as a BMP9-inducible chondroprogenitor.
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影响因子：
2.7
作者：
Ferrari, D;Kosher, RA
通讯作者：
Kosher, RA
DOI：
10.1002/reg2.81
发表时间：
2017-06
期刊：
Regeneration (Oxford, England)
影响因子：
--
作者：
Dawson LA;Yu L;Yan M;Marrero L;Schanes PP;Dolan C;Pela M;Petersen B;Han M;Muneoka K
通讯作者：
Muneoka K
DOI：
10.1126/science.aaq0681
发表时间：
2018-10-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gerber T;Murawala P;Knapp D;Masselink W;Schuez M;Hermann S;Gac-Santel M;Nowoshilow S;Kageyama J;Khattak S;Currie JD;Camp JG;Tanaka EM;Treutlein B
通讯作者：
Treutlein B
影响因子：
4.8
作者：
Gaddy-Kurten, D;Coker, JK;Manolagas, SC
通讯作者：
Manolagas, SC
影响因子：
5.8
作者：
Cai, James J.
通讯作者：
Cai, James J.