Bio-inspired hydrogel composed of hyaluronic acid and alginate as a potential bioink for 3D bioprinting of articular cartilage engineering constructs
Bio-inspired hydrogel composed of hyaluronic acid and alginate as a potential bioink for 3D bioprinting of articular cartilage engineering constructs
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DOI：
10.1016/j.actbio.2020.01.046
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发表时间：
2020-04-01
影响因子：
9.7
通讯作者：
Antonio Marchal, Juan
中科院分区：
文献类型：
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作者：
Antich, Cristina;de Vicente, Juan;Antonio Marchal, Juan
生物打印是一种很有前途的工具，可以制造组织良好的细胞负载结构，用于关节软骨的修复和再生。在组成和机械性能方面选择合适的生物墨水对于开发可行的软骨替代品至关重要。在这项研究中，我们专注于使用软骨的主要成分之一透明质酸（HA）来设计和配制一种用于软骨组织3D生物打印的新生物墨水。分析了该应用所需的主要特性，如可印刷性、生物相容性和生物降解性。为了生产具有最佳机械性能的软骨结构，将HA基生物墨水与聚乳酸（PLA）共印刷。发现基于HA的生物墨水通过增加软骨形成基因标记物的表达和特异性基质沉积以及因此的组织形成来改善细胞功能。这些结果表明，它是一个有前途的生物墨水候选人软骨组织工程的基础上3D bioprinting.Statement的显著性最近出现的3D打印技术已经使巨大的进步，在治疗骨软骨疾病的软骨组织结构的制造，恢复和/或再生受损组织。在这种尝试中，选择合适的生物材料，在组成和机械性能方面，是至关重要的。在这项研究中，我们首次描述了基于软骨主要成分HA的生物墨水的开发，具有合适的生物和机械特性，不涉及毒性过程，及其在软骨组织生物打印中的应用。通过共同打印这种生物墨水和热塑性聚合物PLA制备的混合构建体为软骨细胞生长和维持以及支持施加在天然组织中的负载力所需的机械性能提供了最佳的生态位。我们强调了这种基于HA的生物墨水在临床竞技场中的翻译潜力。(C)2020 Acta Materialia Inc.爱思唯尔有限公司出版
Bioprinting is a promising tool to fabricate well-organized cell-laden constructs for repair and regeneration of articular cartilage. The selection of a suitable bioink, in terms of composition and mechanical properties, is crucial for the development of viable cartilage substitutes. In this study, we focused on the use of one of the main cartilage components, hyaluronic acid (HA), to design and formulate a new bioink for cartilage tissue 3D bioprinting. Major characteristics required for this application such as printability, biocompatibility, and biodegradability were analyzed. To produce cartilage constructs with optimal mechanical properties, HA-based bioink was co-printed with polylactic acid (PLA). HA-based bioink was found to improve cell functionality by an increase in the expression of chondrogenic gene markers and specific matrix deposition and, therefore, tissue formation. These results indicate that it is a promising bioink candidate for cartilage tissue engineering based in 3D bioprinting.Statement of SignificanceThe recent appearance of 3D printing technology has enabled great advances in the treatment of osteochondral disorders by fabrication of cartilage tissue constructs that restore and/or regenerate damaged tissue. In this attempt, the selection of a suitable biomaterial, in terms of composition and mechanical properties, is crucial. In this study, we describe for first time the development of a bioink based on the main component of cartilage, HA, with suitable biological and mechanical properties, without involving toxic procedure, and its application in cartilage tissue bioprinting. Hybrid constructs prepared by co-printing this bioink and thermoplastic polymer PLA provided an optimal niche for chondrocyte growth and maintenance as well as mechanical properties necessary to support load forces exerted in native tissue. We highlight the translation potential of this HA-based bioink in the clinical arena. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd.