A Mobile Secretory Vesicle Cluster Involved in Mass Transport from the Golgi to the Plant Cell Exterior
A Mobile Secretory Vesicle Cluster Involved in Mass Transport from the Golgi to the Plant Cell Exterior
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DOI：
10.1105/tpc.108.058933
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发表时间：
2009-04-01
期刊：
影响因子：
11.6
通讯作者：
Matsuoka, Ken
中科院分区：
文献类型：
--
作者：
Toyooka, Kiminori;Goto, Yumi;Matsuoka, Ken
分泌蛋白和胞外多糖在细胞生长过程中被运输到胞外空间。这些物质被携带在跨高尔基网络(TGN)产生的分泌囊中。对哺乳动物高尔基体后分泌途径的分析表明，分离的分泌小泡在细胞内运动。以分泌载体膜蛋白2(SCAMP2)作为分泌囊泡的标志物，以烟草BY-2细胞为模型细胞，对植物细胞的运输机制进行了研究。结合分析，包括快速冷冻细胞的电子显微镜和表达荧光标记的SCAMP2的细胞的四维分析，能够识别TGN产生的分泌小泡的簇状结构，TGN在细胞中移动并最终与质膜融合。这种结构被称为分泌囊泡团(SVC)。在拟南芥和水稻细胞中也发现了SVC，并在烟草细胞分裂过程中移动到细胞板。因此，在植物细胞中，SVC是一种运动结构，参与从高尔基体到质膜和细胞板的质量运输。
Secretory proteins and extracellular glycans are transported to the extracellular space during cell growth. These materials are carried in secretory vesicles generated at the trans-Golgi network (TGN). Analysis of the mammalian post-Golgi secretory pathway demonstrated the movement of separated secretory vesicles in the cell. Using secretory carrier membrane protein 2 (SCAMP2) as a marker for secretory vesicles and tobacco (Nicotiana tabacum) BY-2 cell as a model cell, we characterized the transport machinery in plant cells. A combination of analyses, including electron microscopy of quick-frozen cells and four-dimensional analysis of cells expressing fluorescent-tagged SCAMP2, enabled the identification of a clustered structure of secretory vesicles generated from TGN that moves in the cell and eventually fuses with plasma membrane. This structure was termed the secretory vesicle cluster (SVC). The SVC was also found in Arabidopsis thaliana and rice (Oryza sativa) cells and moved to the cell plate in dividing tobacco cells. Thus, the SVC is a motile structure involved in mass transport from the Golgi to the plasma membrane and cell plate in plant cells.