Fe-56 ion irradiation induced apoptosis through Nrf2 pathway in mouse testis
Fe-56 ion irradiation induced apoptosis through Nrf2 pathway in mouse testis
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Fe-56离子照射通过Nrf2途径诱导小鼠睾丸细胞凋亡
DOI：
10.1016/j.lfs.2016.05.043
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发表时间：
2016
期刊：
影响因子：
6.1
通讯作者：
Zhang Hong
中科院分区：
文献类型：
--
作者：
Zhao Qiuyue;Mao Aihong;Yan Jiawei;Si Jing;Zhou Rong;Gan Lu;Liu Yang;Zhang Hong
由于太空任务期间暴露于高 LET 辐射的可能性很高，这种现象引起了人们对延长载人深空任务安全性的担忧。 56Fe等重离子是深空的主要辐射源，可能对太空飞行人员在任务期间和任务结束后构成重大危险。由于睾丸是辐射敏感器官，可能容易受到空间辐射引起的变化的影响。在本研究中，我们研究了56Fe辐照对小鼠睾丸的影响和潜在机制。使用 0.5 和 1 Gy56Fe 辐射进行全身照射后测量病理特征。采用流式细胞术和末端 dUTP 缺口末端标记 (TUNEL) 来检测凋亡细胞。应用蛋白质印迹来鉴定潜在的生物标志物。免疫荧光用于研究蛋白质定位。我们发现1 Gy组的病理变化和凋亡细胞明显高于0 Gy组。此外，蛋白质表达和定位研究证实Nrf2参与了这种急性损伤。 Nrf2 及其靶基因 HO-1 和 NQO1 在受辐射的睾丸中以剂量依赖性方式上调。 Nrf2可能是辐射诱导的细胞反应中有用的分子标记，并且对于检测暴露于空间辐射后的异常精子发生很重要。
The phenomenon has raised the concerns about the safety of an extended manned mission into deep space due to the high potential for exposure to high-LET radiation during space missions. Heavy ions such as56Fe are main radiation sources in deep space, which could pose a significant hazard to space flight crews during and after missions. Since the testis is a radiosensitive organ, which may be susceptible to space radiation-induced changes. In this study, we investigated the effect and potential mechanisms of56Fe irradiation on mouse testis. Pathological characteristics were measured following whole-body irradiation with 0.5 and 1 Gy56Fe irradiation. Flow cytometry and terminal dUTP nick end-labeling (TUNEL) were performed to detect apoptotic cells. Western blot was applied to identify potential biomarkers. Immunofluorescence was used to investigate protein localization. We found that pathologic changes and apoptosis cells were significantly higher in 1 Gy group than those in 0 Gy groups. In addition, protein expression and localization studies confirmed Nrf2 was involved in this acute injury. Nrf2 and its target genes HO-1 and NQO1 were up-regulated in the irradiated testis in a dose-dependent manner. Nrf2 may be useful molecular markers in radiation-induced cellular responses and is important for detecting abnormal spermatogenesis following exposure to space radiation.