Actin is involved in auxin-dependent patterning
Actin is involved in auxin-dependent patterning
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DOI：
10.1104/pp.106.094052
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发表时间：
2007-04-01
期刊：
影响因子：
7.4
通讯作者：
Nick, Peter
中科院分区：
文献类型：
--
作者：
Maisch, Jan;Nick, Peter
生长素的极性运输已被认为是模式形成的中心要素。生长素运输的极性与针状蛋白的循环有关，这一过程与肌动球蛋白依赖的囊泡运输有关。为了深入了解肌动蛋白在生长素运输中的作用，我们使用图案化的细胞分裂来监测生长素通量的极性。我们发现烟草细胞系的细胞分裂是部分同步的，这种同步性可以被1-N-萘基邻苯二甲酸抑制生长素运输所干扰。为了解决肌动蛋白在这种同步性中的作用，我们通过过度表达小鼠talin诱导了肌动蛋白的捆绑配置。肌动蛋白的捆绑破坏了细胞分裂的同步性，增加了细胞对1-N-萘基邻苯二甲酸的敏感性。加入极性运输的生长素吲哚-3-乙酸和1-萘乙酸(而不是2，4-二氯苯氧乙酸)，既恢复了肌动蛋白的正常组织，又恢复了细胞分裂的同步性。这项研究表明，生长素通过改变肌动蛋白细丝的状态来控制自己的运输。
Polar transport of auxin has been identified as a central element of pattern formation. The polarity of auxin transport is linked to the cycling of pin-formed proteins, a process that is related to actomyosin-dependent vesicle traffic. To get insight into the role of actin for auxin transport, we used patterned cell division to monitor the polarity of auxin fluxes. We show that cell division in the tobacco (Nicotiana tabacum L. cv Bright-Yellow 2) cell line is partially synchronized and that this synchrony can be perturbed by inhibition of auxin transport by 1-N-naphthylphthalamic acid. To address the role of actin in this synchrony, we induced a bundled configuration of actin by overexpressing mouse talin. The bundling of actin impairs the synchrony of cell division and increases the sensitivity to 1-N-naphthylphthalamic acid. Addition of the polarly transported auxins indole-3-acetic acid and 1-naphthyl acetic acid (but not 2,4-dichlorophenoxyacetic acid) restored both the normal organization of actin and the synchrony of cell division. This study suggests that auxin controls its own transport by changing the state of actin filaments.