High-resolution analysis of the spatio-temporal activity patterns in rat olfactory bulb evoked by enantiomer odors.
High-resolution analysis of the spatio-temporal activity patterns in rat olfactory bulb evoked by enantiomer odors.
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DOI：
10.1093/chemse/28.6.499
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发表时间：
2003-07
期刊：
影响因子：
3.5
通讯作者：
M. Lehmkuhle;R. Normann;E. Maynard
中科院分区：
文献类型：
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作者：
M. Lehmkuhle;R. Normann;E. Maynard
了解哺乳动物如何处理嗅觉刺激促进了工具和技术的发展，这些工具和技术允许同时研究神经活动的精细结构的空间和时间模式。描述了一种技术，该技术使用 32 个穿透性微电极阵列植入大鼠嗅球的两侧，以研究二尖瓣和簇状神经元对多种浓度的简单对映体气味对刺激的反应。结果表明，最多可对 49 个单机和多机进行稳定、同步的记录，持续时间长达 14 小时。我们表明，这种气味会引起独特的空间和快速时间活动模式，这可能有助于气味辨别。该技术可扩展到嗅觉感觉处理的其他系统神经科学研究。
Understanding how mammals process olfactory stimuli has motivated the development of tools and techniques which permit the simultaneous study of finely structured spatial and temporal patterns of neural activity. A technique is described that uses an array of 32 penetrating microelectrodes implanted bilaterally into the dorsal aspect of rat olfactory bulb to investigate the responses of mitral and tufted neurons to stimulation with simple enantiomer odor pairs at a number of concentrations. It is shown that stable, simultaneous recordings from up to 49 single- and multi-units can be performed for periods of up to 14 h. We show that such odors evoke unique spatial and fast-temporal activity patterns which may subserve odor discrimination. This technique is extensible to other systems neuroscience investigations of olfactory sensory processing.