Cytotoxicity test and mass spectrometry of IPMC
Cytotoxicity test and mass spectrometry of IPMC
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DOI：
10.1002/ecj.10239
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发表时间：
2008
影响因子：
0.3
通讯作者：
Kazuto Takashima;N. Kamamichi;T. Yagi;K. Asaka;T. Mukai
中科院分区：
文献类型：
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作者：
Kazuto Takashima;N. Kamamichi;T. Yagi;K. Asaka;T. Mukai
离子聚合物-金属复合材料（IPMC）是生物医学致动器和传感器中很有前途的材料，因为它们柔软而柔韧，从而确保了设备本身的安全性。本研究的目的是通过体外实验研究IPMC的生物相容性，以评估其在生物医学领域的适用性。除了通过使用阳离子金络合物和还原剂的常规“吸附-还原法”制备的IPMC试样之外，还通过另外的过程制备了两个试样：一个试样在Na 2SO 3溶液中还原，另一个在H2 O2溶液中清洁。使用中国仓鼠V79细胞的集落形成试验显示所有IPMC标本均具有高细胞毒性。通过直接入口质谱法（DI-MS）的检查显示，金络合物的峰强度（特别是，m/z=180）与Nafion膜的峰强度不同。监测m/z=180处的峰显示在未在H2 O2溶液中清洗的IPMC样品中残留有邻菲咯啉结构。© 2009威利期刊公司. Electron Comm Jpn，93（1）：1-8，2010;在线发表于Wiley InterScience（www.interscience.wiley.com）。DOI 10.1002/ecj.10239
Ionic polymer-metal composites (IPMC) are promising materials in biomedical actuators and sensors, as they are soft and flexible, thus assuring the safety of the device itself. The purpose of this study is to investigate the biocompatibility of IPMC in in vitro experiments in order to evaluate their applicability in biomedical fields. In addition to an IPMC specimen prepared by the conventional “impregnation-reduction method” using cationic gold complexes and reducing agents, two specimens were prepared by additional processes: one specimen was reduced in Na2SO3 solution and the other was cleaned in H2O2 solution. A colony-forming test using Chinese hamster V79 cells showed high cytotoxicity for all IPMC specimens. Examination by direct inlet mass spectrometry (DI-MS) revealed that the peak intensity of the gold complex (particularly, m/z=180) was different from that of Nafion film. Monitoring the peak at m/z=180 showed a remnant with the structure of phenanthroline in IPMC specimens that were not cleaned in H2O2 solution. © 2009 Wiley Periodicals, Inc. Electron Comm Jpn, 93(1): 1–8, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecj.10239