Evidence for complex formation between GTP binding protein(Gs) and membrane-associated nucleoside diphosphate kinase.
Evidence for complex formation between GTP binding protein(Gs) and membrane-associated nucleoside diphosphate kinase.
复制标题
GTP 结合蛋白 (Gs) 和膜相关核苷二磷酸激酶之间形成复合物的证据。
DOI：
10.1016/0006-291x(90)91680-q
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
N. Shimada
中科院分区：
文献类型：
--
作者：
Narimichi Kimura;N. Shimada
当大鼠肝膜中的Gs预先用[32P]NAD和霍乱毒素标记，用辛基葡萄糖苷溶解，然后通过蔗糖密度梯度离心分析时，它被分成两个分子大小近似为12-13S和3-4S的峰。对标记膜进行不加或加 GDPβS 的预处理会在高分子量区域产生较大的峰，而用胰高血糖素加 GTPτS 进行预处理会在两个区域产生几乎相同的峰。亲和纯化的抗核苷二磷酸 (NDP) 激酶抗体仅沉淀高分子量区域的 G。在相同条件下，小但显着的 NDP 激酶活性与高分子量 Gs 区域相关，尽管大部分酶活性在其单独出现的部分中恢复 (6.2S)。 Lubrol-PX 和洋地黄皂苷均以对抗 NDP 激酶抗体的免疫沉淀不敏感的形式溶解 G，尽管后者去污剂能够以高分子量形式（即三元胰高血糖素-受体-G 蛋白复合物）溶解 G。这些结果表明，Gs 和膜相关 NDP 激酶可能部分以复合形式存在于膜中。讨论了膜信号转导中复合物形成的生理相关性。
When the Gs in rat liver membranes was prelabeled with [32P]NAD and cholera toxin, solubilized with octylglucoside, and then analyzed by sucrose density gradient centrifugation, it was fractionated into two peaks with approximate molecular sizes of 12–13S and 3–4S. Pretreatment without or with GDPβS of the labeled membranes resulted in a larger peak in the high molecular weight region, whereas pretreatment with glucagon plus GTPτS caused almost equal peaks in both regions. The affinity-purified anti-nucleoside diphosphate(NDP) kinase antibodies only precipitated the Gs in high molecular weight region. Under the same condition, small but significant NDP kinase activity was associated with the high molecular weight Gs region although a large portion of the enzyme activity was recovered in fractions where it alone should appear(6.2S). Both Lubrol-PX and digitonin solubilized the Gs in forms insensitive to immunoprecipitation by anti-NDP kinase antibodies although the latter detergent was able to solubilize the Gs in a high molecular weight form, that is, a ternary glucagon-receptor-G protein complex. These results demonstrate that Gs and membrane-associated NDP kinase may exist in part in a complexed form in membranes. Physiological relevance of the complex formation in membrane signal transduction is discussed.
腺苷酸环化酶的抑制性鸟嘌呤核苷酸结合调节成分的纯化和特性。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bokoch,GM;Katada,T;Northup,JK;Ui,M;Gilman,AG
通讯作者：
Gilman,AG