Cationic liposome and plasmid DNA complexes formed in serum-free medium under optimum transfection condition are negatively charged
Cationic liposome and plasmid DNA complexes formed in serum-free medium under optimum transfection condition are negatively charged
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DOI：
10.1016/s0005-2736(00)00176-0
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发表时间：
2000-06-01
影响因子：
3.4
通讯作者：
Park, A
中科院分区：
文献类型：
--
作者：
Son, KK;Patel, DH;Park, A
在常规进行体外转染的培养基中。单独的DC-chol脂质体几乎是中性的，而DC-chol脂质体/DNA复合物在很大程度上带负电荷，在所有[脂质体]/[DNA]比率下仅轻微变化（zeta = -27.1至-21.8 mV）。其它三种商业转染试剂，Lipofectin（（R））、LipofectAMINE（TM）2000和SuperFect（TM），当与DNA复合时也大部分带负电荷。通过加入DNA来防止脂质体在培养基中的聚集。在培养基中孵育的复合物没有改变它们的大小，电荷或脂转染活性为30分钟。这些结果表明，在介质中，脂质体/DNA复合物形成的时间与负电荷混合。(C)2000爱思唯尔科技有限公司。保留所有权利。
In medium where in vitro transfection is routinely performed. DC-chol liposomes alone were nearly neutral, whereas the DC-chol liposome/DNA complexes were largely negatively charged which changed only slightly at all [liposome]/[DNA] ratios (zeta = -27.1 to -21.8 mV). Three other commercial transfection reagents, Lipofectin((R)), LipofectAMINE((TM)) 2000, and SuperFect((TM)), were also largely negatively charged when complexed with DNA. The aggregation of liposomes in medium was prevented by the addition of DNA. Incubation of the complexes in medium did not change their size, charge or lipofection activity for 30 min. These results suggest that, in medium, the liposome/DNA complexes were formed at the time of mixing with negative charges. (C) 2000 Elsevier Science B.V. All rights reserved.