POSITIVE SELECTION FOR LOSS OF TETRACYCLINE RESISTANCE
POSITIVE SELECTION FOR LOSS OF TETRACYCLINE RESISTANCE
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DOI：
10.1128/jb.143.2.926-933.1980
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发表时间：
1980-01-01
影响因子：
3.2
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
BOCHNER, BR;HUANG, HC;AMES, BN
设计了一种简单的技术，允许直接从主要四环素耐药[大肠杆菌和鼠伤寒沙门氏菌]群体中选择四环素敏感克隆。该技术在基于使用四环素抗性转座子（如Tn10）的遗传方法学中特别有用。该方法的潜在用途包括缺失突变体的选择、精细结构作图、广义作图、多重标记菌株的构建、四环素抗性转座子和质粒的消除以及克隆。该技术是基于四环素耐药细胞对亲脂性螯合剂（如镰刀菌酸）超敏感的发现。显然，某些金属离子对四环素的摄取有重要的促进作用。四环素耐药的分子机制提出了建议。
A simple technique was devised that allows direct plate selection of tetracycline-sensitive clones from a predominantly tetracycline-resistant [Escherichia coli and Salmonella typhimurium] population. The technique is especially useful in genetic methodologies based on the use of tetracycline resistance transposons, such as Tn10. Potential uses of the method include selection of deletion mutants, fine-structure mapping, generalized mapping, construction of multiply marked strains, elimination of tetracycline resistance transposons and plasmids, and cloning. The technique is based on the finding that tetracycline-resistant cells are hypersensitive to lipophilic chelating agents, such as fusaric acid. Apparently certain metal ions critically facilitate tetracycline uptake. Possible molecular mechanisms for tetracycline resistance are suggested.