Expression of NPR-B in neurons of the dorsal root ganglia of the rat
Expression of NPR-B in neurons of the dorsal root ganglia of the rat
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DOI：
10.1016/j.peptides.2013.02.013
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发表时间：
2013-05-01
期刊：
影响因子：
3
通讯作者：
Tooyama, Ikuo
中科院分区：
文献类型：
--
作者：
Abdelalim, Essam M.;Bellier, Jean-Pierre;Tooyama, Ikuo
C型利钠肽（CNP）是中枢神经系统中含量丰富的一种神经肽，通过利钠肽受体B（NPR-B）发挥其生理作用。最近，CNP/NPR-B系统已被认为是感觉轴突发育的重要调节因子。背根神经节（dorsal root ganglion，DRG）是一个将多种脊髓感觉刺激传入中枢神经系统的神经元。在这项研究中，我们的特点是NPR-B受体在大鼠DRG的表达，使用逆转录聚合酶链反应，蛋白质印迹和免疫组织化学。免疫组化显示NPR-B在DRG的神经元胞体和突起中表达，主要在DRG的小型和中型神经元中表达。免疫双标记显示，NPR-B在降钙素基因相关肽和异凝集素B4阳性神经元中表达。此外，NPR-B的表达与降钙素基因相关肽在脊髓背角共定位。总之，我们的数据表明，利钠肽可能通过与DRG中的NPR-B结合对感觉神经元执行几种生物学作用。(C)2013 Elsevier Inc. All rights reserved.
C-type natriuretic peptide (CNP) is an abundant neuropeptide in the central nervous system, which exerts its physiological effects through natriuretic peptide receptor B (NPR-B). Recently, the CNP/NPR-B system has been recognized as an important regulator for the development of sensory axons. The dorsal root ganglion (DRG) contains neurons transmitting several kinds of spinal sensory stimuli to the central nervous system. In this study, we characterized NPR-B receptor expression in the rat DRG, using reverse transcription-polymerase chain reaction, Western blotting and immunohistochemistry. Immunostaining revealed that NPR-B was expressed in neuronal cell bodies and processes of the DRG, with NPR-B immunoreactivity mainly prominent in small and medium-sized DRG neurons. Double-immunolabeling showed that NPR-B was expressed in calcitonin gene-related peptide- and isolectin B4-positive neurons. Furthermore, NPR-B expression was co-localized with calcitonin gene-related peptide in the dorsal horn of the spinal cord. Together, our data suggest that the natriuretic peptides may perform several biological actions on sensory neurons via their binding to NPR-B in the DRG. (C) 2013 Elsevier Inc. All rights reserved.