Effects of bone marrow-derived mesenchymal stem cell transplantation in piglet Legg-Calve-Perthes disease models: a pilot study
Effects of bone marrow-derived mesenchymal stem cell transplantation in piglet Legg-Calve-Perthes disease models: a pilot study
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DOI：
10.1097/bpb.0000000000001095
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发表时间：
2024-07-01
影响因子：
1.1
通讯作者：
Mishima，Hajime
中科院分区：
文献类型：
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作者：
Tomaru，Yohei;Sugaya，Hisashi;Mishima，Hajime
本初步研究探讨了骨髓间充质干细胞移植治疗仔猪Legg-Calve-Perthes病（LCPD）模型的有效性和安全性。通过结扎股骨颈，在两只长白猪仔猪（6周龄和7周龄，体重分别为12 kg和17 kg）中诱导LCPD模型。在第一头仔猪中，观察到自然LCPD过程。第2组于结扎股骨颈4周后，将单纯培养液和含2.44× 107骨髓间充质干细胞的培养液分别移植于髓芯减压后的左右股骨头内。每4周拍摄一次X线平片，以最大骨骺高度除以最大骨骺直径计算骨骺商。术后14周处死仔猪。取出股骨头并进行大体、病理和计算机断层扫描评价。采用流式细胞术评价移植细胞的特性。在第一只仔猪的两个股骨头中观察到骨骺变平，仅在第二只仔猪的右髋中观察到骨骺变平。术后即刻和14周，第2只仔猪右侧股骨头的骨骺比分别为0.40和0.14，而左侧股骨头的骨骺比分别为0.30和0.42。苏木精和伊红染色未显示骨骺棒或肿瘤细胞形成。移植细胞的CD 44、CD 105、CD 29和CD 31阳性率分别为99.2%、65.9%、18.2%和0.16%。髓芯减压联合骨髓间充质干细胞移植预防骨骺塌陷。
This preliminary study investigated the efficacy and safety of bone marrow-derived mesenchymal stem cell transplantation in a piglet Legg–Calve–Perthes disease (LCPD) model. The LCPD model was induced in two Landrace piglets (6-and 7-week-old, weighing 12 and 17 kg, respectively) by ligaturing the femoral neck. In the first piglet, the natural LCPD course was observed. In the second piglet, 4 weeks after ligaturing the femoral neck, simple medium and medium containing 2.44× 10 7 bone marrow-derived mesenchymal stem cells were transplanted into the right and left femoral heads after core decompression, respectively. Plain radiographs were obtained every 4 weeks, and the epiphyseal quotient was calculated by dividing the maximum epiphysis height by the maximum epiphysis diameter. The piglets were sacrificed at 14 weeks postoperatively. The femoral heads were extracted and evaluated grossly, pathologically, and by using computed tomography. The transplanted cell characteristics were evaluated using flow cytometry. Flattening of the epiphysis was observed in both femoral heads of the first piglet and only in the right hip of the second piglet. The epiphyseal quotients immediately and at 14 weeks postoperatively in the right femoral head of the second piglet were 0.40 and 0.14, respectively, while those of the left femoral head were 0.30 and 0.42, respectively. Hematoxylin and eosin staining did not reveal physeal bar or tumor cell formation. The transplanted cells were 99.2%, 65.9%, 18.2%, and 0.16% positive for CD44, CD105, CD29, and CD31, respectively. Core decompression combined with bone marrow-derived mesenchymal stem cell transplantation prevented epiphyseal collapse.