Sequencing-based gene network analysis provides a core set of gene resource for understanding thermal adaptation in Zhikong scallop Chlamys farreri
Sequencing-based gene network analysis provides a core set of gene resource for understanding thermal adaptation in Zhikong scallop Chlamys farreri
复制标题
基于测序的基因网络分析为理解直孔扇贝栉孔扇贝热适应提供了一套核心基因资源
DOI：
10.1111/1755-0998.12169
复制
发表时间：
2014-01-01
影响因子：
7.7
通讯作者：
Bao, Z.
中科院分区：
文献类型：
--
作者：
Fu, X.;Sun, Y.;Bao, Z.
海洋生物通常暴露在多变的环境条件下，其中许多海洋生物受到全球气候变化导致的海洋温度上升的威胁。在不同的胁迫条件下产生转录转录资源对于理解热适应的分子机制是至关重要的。在这项研究中，我们对受到急性和慢性热应激的栉孔扇贝进行了全转录组的基因表达谱分析。在13953个唯一标签中，有850多个标签在急性热应激后的各个时间点有显著差异表达，超过了慢性热应激下差异表达的标签数量(320-350个)。为了系统地了解热应激过程中基因表达的变化，构建了一个加权的基因共表达网络。有6个模块被鉴定为急性热应激反应模块。其中，参与细胞凋亡调控、mRNA结合、线粒体膜形成和氧化还原的4个模块表达下调。其余两个模块已上调。其中一个富含伴侣，另一个富含微卫星序列，它们的共表达可能源于转录因子结合位点。这些结果表明，华支睾吸虫触发了几个适应高温的细胞过程。没有模块对慢性热应激做出反应，这表明扇贝可能在3天内适应了高温。本研究首次在非模式水生生物中进行了基于测序的基因网络分析，为热适应研究提供了有价值的基因资源，有助于培育耐高温的水产养殖扇贝品系。
Marine organisms are commonly exposed to variable environmental conditions, and many of them are under threat from increased sea temperatures caused by global climate change. Generating transcriptomic resources under different stress conditions are crucial for understanding molecular mechanisms underlying thermal adaptation. In this study, we conducted transcriptome-wide gene expression profiling of the scallop Chlamys farreri challenged by acute and chronic heat stress. Of the 13953 unique tags, more than 850 were significantly differentially expressed at each time point after acute heat stress, which was more than the number of tags differentially expressed (320-350) under chronic heat stress. To obtain a systemic view of gene expression alterations during thermal stress, a weighted gene coexpression network was constructed. Six modules were identified as acute heat stress-responsive modules. Among them, four modules involved in apoptosis regulation, mRNA binding, mitochondrial envelope formation and oxidation reduction were downregulated. The remaining two modules were upregulated. One was enriched with chaperone and the other with microsatellite sequences, whose coexpression may originate from a transcription factor binding site. These results indicated that C.farreri triggered several cellular processes to acclimate to elevated temperature. No modules responded to chronic heat stress, suggesting that the scallops might have acclimated to elevated temperature within 3days. This study represents the first sequencing-based gene network analysis in a nonmodel aquatic species and provides valuable gene resources for the study of thermal adaptation, which should assist in the development of heat-tolerant scallop lines for aquaculture.