Circular Herpesvirus sylvilagus DNA in spleen cells of experimentally infected cottontail rabbits
Circular Herpesvirus sylvilagus DNA in spleen cells of experimentally infected cottontail rabbits
复制标题
实验感染棉尾兔脾细胞中的圆形疱疹病毒 syvilagus DNA
作者：
P. Medveczky;W. Kramp;J. Sullivan
Cottontail rabbits (Sylvilagus floridanus) were infected with Herpesvirus sylvilagus, and spleen cells were analyzed for the presence of virus-specific, covalently closed circular, and linear DNA molecules by a simple electrophoretic technique, followed by transfer to nitrocellulose filters and hybridization with cloned viral DNA (Gardella et al., J. Virol. 50:248-254, 1984). Approximately 0.2 copies per cell of circular DNA and 0.2 copies per cell of linear DNA were detected by hybridization with a cloned viral DNA fragment. The size of the viral DNA was estimated at ca. 158 kilobase pairs. Restriction endonuclease patterns suggested structural similarities to cottontail herpesvirus DNA.
