Dichlorvos poisoning caused chicken cerebrum tissue damage and related apoptosis-related gene changes.
Dichlorvos poisoning caused chicken cerebrum tissue damage and related apoptosis-related gene changes.
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DOI：
10.1016/j.scitotenv.2021.147051
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
Yueming Gu;Guyue Li;Cheng Huang;Pei Liu;G. Hu;Cong Wu;Zheng Xu;Xiaoquan Guo;Ping Liu
中科院分区：
文献类型：
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作者：
Yueming Gu;Guyue Li;Cheng Huang;Pei Liu;G. Hu;Cong Wu;Zheng Xu;Xiaoquan Guo;Ping Liu
敌敌畏（DDVP）是一种具有杀虫作用的有机磷化合物。有机磷农药极易通过各种渠道进入人体或动物体内，造成大脑神经细胞损伤。本研究旨在探讨急性敌敌畏中毒是否会引起肉鸡大脑神经毒性损伤及细胞凋亡相关基因表达的改变，进一步明确急性敌敌畏暴露后的神经毒性机制，为后期的预防、治疗及基因药物筛选提供研究依据。本试验将健康黄羽肉鸡随机分为对照组、低剂量组（1.13 mg/kg）和高剂量组（10.2 mg/kg）进行模型观察，采用血红素和伊红染色、组织切片透射电镜分析、免疫荧光技术和实时定量聚合酶链反应（qRT-PCR）进行检测。结果显示，有机磷中毒患者伴有肢体抽搐、大量流涎等明显的神经系统症状。此外，我们观察到，与对照组相比，中毒组肉鸡核神经元裂解数量、血管鞘变形数量、神经胶质细胞数量及神经胶质纤维酸性蛋白（GFAP）表达均显著增加，且低剂量组增加更为明显。然而，高剂量组细胞凋亡和线粒体结构溶解最为明显。此外，qRT-PCR结果也显示凋亡相关基因的表达发生了显著变化。facc、lkb1、gpat1表达量显著升高，hmgr、PPARα、cpt1、ampk α1表达量显著降低。综上所述，这些结果表明，敌敌畏可能引起大脑神经细胞核的裂解，彻底破坏线粒体结构，改变相关凋亡基因的表达，增强细胞凋亡，对大脑造成神经源性损伤。这些研究结果为急性有机磷中毒的防治提供了理论依据。
Dichlorvos (DDVP) is an organophosphorus compound with insecticidal effects. Organophosphorus pesticides can easily enter humans or animals through various channels, causing cerebrum nerve cell damage. The purpose of this research was to investigate whether acute dichlorvos poisoning can cause cerebrum neurotoxic injury and change the expression of apoptosis-related genes in broilers, further clarify the neurotoxic mechanism after acute dichlorvos exposure, and provide a research basis for prevention, treatment and gene drug screening in the later stage. In this experiment, healthy yellow-feathered broilers were randomly assigned to the control group, the low-dose group (1.13 mg/kg) and the high-dose group (10.2 mg/kg) for modelling observation, and detection was conducted based on H&E (haematoxylin and eosin) staining, transmission electron microscopy analysis of tissue sections, immunofluorescence techniques and real-time quantitative polymerase chain reaction (qRT-PCR). The results showed that organophosphorus poisoning was accompanied by obvious neurological symptoms such as limb twitching and massive salivation. In addition, we observed that compared with the control group, the number of lysed nuclear neurons, deformed vascular sheaths, and glial cells and the expression of glial fibrillary acidic protein (GFAP) in the poisoned group of broilers increased significantly, and the increase was more obvious in the low-dose group. However, cell apoptosis and mitochondrial structure dissolution were most pronounced in the high-dose group. Moreover, the qRT-PCR results also revealed significant changes in the expression of apoptosis-related genes. The expression levels ofACC,LKB1andGPATincreased significantly, while the expression ofHMGR,PPARα,CPT1andAMPKα1decreased significantly. In summary, these results indicated that dichlorvos may cause the lysis of cerebrum nerve cell nuclei, completely destroy the structure of mitochondria, change the expression of related apoptotic genes, enhance cell apoptosis, and cause neurogenic damage to the cerebrum. These research results offer a theoretical foundation for the prevention and treatment of acute organophosphate toxicosis.