Fab fragments from a monoclonal antibody against a germ tube mannoprotein block the yeast-to-mycelium transition in Candida albicans.
Fab fragments from a monoclonal antibody against a germ tube mannoprotein block the yeast-to-mycelium transition in Candida albicans.
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针对芽管甘露糖蛋白的单克隆抗体的 Fab 片段可阻断白色念珠菌中酵母菌向菌丝体的转变。
DOI：
10.1128/iai.58.11.3810-3812.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
Chaffin,WL
中科院分区：
文献类型：
--
作者：
Casanova,M;Martínez,JP;Chaffin,WL
由免疫球蛋白G单克隆抗体（MAb） 4C12制备的Fab片段，与白色念珠菌芽管菌丝延伸上表达的决定因子反应，抑制芽管的形成，但完整的MAb 4C12没有。间接免疫荧光显示，与Fab片段孵育的细胞呈点状结合模式，而与完整MAb 4C12孵育的细胞呈融合结合模式。
Fab fragments prepared from the immunoglobulin G monoclonal antibody (MAb) 4C12, which reacts with a determinant expressed on the hyphal extension of germ tubes of Candida albicans, inhibited germ tube formation, but intact MAb 4C12 did not. Indirect immunofluorescence showed a punctate binding pattern on cells incubated with Fab fragments but a confluent binding on cells incubated with intact MAb 4C12.