An intracellular HRP study of the rat globus pallidus. II. Fine structural characteristics and synaptic connections of medially located large GP neurons.
An intracellular HRP study of the rat globus pallidus. II. Fine structural characteristics and synaptic connections of medially located large GP neurons.
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大鼠苍白球的细胞内 HRP 研究。
DOI：
10.1002/cne.902210210
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Kitai,ST
中科院分区：
文献类型：
--
作者：
Falls,WM;Park,MR;Kitai,ST
为了分类的突触前元件接触的主要类苍白球神经元，电子显微镜检查的连续切片从一个位于中间的大苍白球神经元，标记与细胞内辣根过氧化物酶，进行了。此外，使用标记和光显微镜重建的材料，使我们能够定量地确定每种终扣类型沿着索马和树突的分布。与标记细胞接触的突触前末梢有六种类型。1型末端，最多（84%），在细胞的所有部分上对称接触，除了刺，包含大的多形性和一些大的致密核心囊泡。2型末端充满了小的球形至椭圆形突触囊泡。它们只与高阶树突进行不对称接触，占标记神经元上突触接触的12%。第三型末端较大，含有稀疏分布的大型多形性小泡，每个终扣形成两个对称的突触，一个在棘头上，另一个在下面的树突轴上。它们是罕见的（0.2%），被发现只与树突棘。4型末端含有大的多形性突触囊泡，没有致密核心囊泡。它们与短的初级树突形成对称的接触。5型末端含有小的透明多形性囊泡和许多大的致密核心囊泡的混合物。它们只接触细胞体和短的初级树突，占体细胞突触接触的20%，但不到树突接触的1%。6型终扣包含椭圆形和扁平的突触囊泡，并与高级树突分支和细胞体建立对称接触。
In order to classify the presynaptic elements contacting the principle class of globus pallidus neurons, electron microscopic examination of serial sections made from a medially located large globus pallidus neuron, labeled with intracellular horseradish peroxidase, was undertaken. In addition, the use of labeled and light microscopically reconstructed material allowed us to quantitatively determine the distribution of each bouton type along the soma and dendrites. Six types of presynaptic terminals contacting the labeled cell have been recognized. Type 1 endings, the most numerous (84%), make symmetrical contacts on all portions of the cell, except spines, contain large pleomorphic, and a few large dense‐core vesicles. Type 2 endings are filled with small spherical‐to‐ellipsoidal synaptic vesicles. They make asymmetrical contacts only with higher‐order dendrites and account for 12% of synaptic contacts onto the labeled neuron. Type 3 endings are large, contain sparsely distributed large pleomorphic vesicles, and make two symmetrical synapses per bouton, one onto a spine head and the other onto the underlying dendritic shaft. They are infrequent (0.2%), being found only in association with dendritic spines. Type 4 endings contain large pleomorphic synaptic vesicles and no dense‐core vesicles. They make symmetrical contacts with the short primary dendrites. Type 5 endings contain a mixture of small clear pleomorphic vesicles and numerous large dense‐core vesicles. They contact only the cell body and the short primary dendrites, making up 20% of somatic synaptic contacts but less than 1% of contacts onto dendrites. Type 6 boutons contain oval and flattened synaptic vesicles and establish symmetrical contacts with higher‐order dendritic branches and the cell body.