Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella enterica from Dairy Cattle Farms in the Wakiso District, Uganda: A Cross-Sectional Study
Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella enterica from Dairy Cattle Farms in the Wakiso District, Uganda: A Cross-Sectional Study
复制标题
DOI：
10.1089/fpd.2018.2528
复制
发表时间：
2019-01-01
影响因子：
2.8
通讯作者：
Fedorka-Cray, Paula J.
中科院分区：
文献类型：
--
作者：
Ball, Takiyah A.;Monte, Daniel F.;Fedorka-Cray, Paula J.
产β-内酰胺酶的肠杆菌科细菌在世界范围内迅速蔓延，对人-畜-环境界面构成严重威胁。在本研究中，我们报告了从乌干达20个农场的400份环境奶牛粪便样本中分离出的肠道沙门氏菌（1.3%）和共生大肠杆菌（96.3%）。在E.大肠杆菌分离株中，21%对至少一种所测试的抗菌剂具有耐药性，7%表现出多药耐药性。四E。大肠埃希菌分离株显示产超广谱β-内酰胺酶（ESBL）的基因，包括bla（CTX-M-15）（n = 2/4）、bla（CTX-M-27）（n = 1/4）、bla（SHV-12）（n = 1/4）和bla（TEM-1B）（n = 2/4）。全基因组测序证实3株ESBL分离株中存在质粒介导的喹诺酮类耐药qnrS 1基因。在季节性流行率方面，E. coli和革兰氏阳性菌S.对各采样期奶牛肠道菌群进行了观察。此外，据我们所知，这是首次报道E.携带从乌干达奶牛中分离的bla（CTX-M-15）、bla（CTX-M-27）、bla（SHV-12）或qnrS 1的大肠杆菌。我们的结论是，全球传播的bla（CTX-M-15）和bla（CTX-M-27）的存在需要进一步研究，以防止进一步传播。此外，耐氟喹诺酮类产ESBL大肠杆菌的存在。奶牛场的大肠杆菌突出了人-畜-环境相互作用中的潜在风险。这项研究可作为在乌干达全境实施更强有力的国家综合监测系统的基准。
Enterobacteriaceae producing beta-lactamases have spread rapidly worldwide and pose a serious threat to human-animal-environment interface. In this study, we present the presence of Salmonella enterica (1.3%) and commensal Escherichia coli (96.3%) isolated from 400 environmental fecal dairy cattle samples over 20 farms in Uganda. Among E. coli isolates, 21% were resistant to at least one antimicrobial tested and 7% exhibited multidrug resistance. Four E. coli isolates displayed extended-spectrum beta-lactamase (ESBL)-producing genes, including bla(CTX-M-15) (n = 2/4), bla(CTX-M-27) (n = 1/4), bla(SHV-12) (n = 1/4), and bla(TEM-1B) (n = 2/4). Whole genome sequencing confirmed the presence of the plasmid-mediated quinolone resistance qnrS1 gene among three ESBL isolates. No statistically significant differences in seasonal prevalence for E. coli and S. enterica among dairy cattle sampling periods were observed. Furthermore, to our knowledge, this is the first report of E. coli carrying bla(CTX-M-15), bla(CTX-M-27), bla(SHV-12), or qnrS1 isolated from dairy cattle in Uganda. We conclude that the presence of globally disseminated bla(CTX-M-15) and bla(CTX-M-27) warrants further study to prevent further spread. In addition, the presence of fluoroquinolone resistant ESBL-producing E. coli on dairy farms highlights the potential risk among the human-livestock-environment interaction. This study can be used as a baseline for implementation of a more robust national integrated surveillance system throughout Uganda.