The accumulation and efflux of lead partly depend on ATP-dependent efflux pump-multidrug resistance protein 1 and glutathione in testis Sertoli cells
The accumulation and efflux of lead partly depend on ATP-dependent efflux pump-multidrug resistance protein 1 and glutathione in testis Sertoli cells
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铅的积累和外排部分依赖于睾丸支持细胞中ATP依赖性外排泵——多药耐药蛋白1和谷胱甘肽
DOI：
10.1016/j.toxlet.2014.02.017
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发表时间：
2014-05-02
影响因子：
3.5
通讯作者：
Wang, Chunhong
中科院分区：
文献类型：
--
作者：
Huang, Shaoxin;Ye, Jingping;Wang, Chunhong
由于铅的积累对细胞有毒，因此其排泄对于生物体在毒性中生存至关重要。本研究以小鼠睾丸支持细胞（TM 4）和多药耐药蛋白1（Mrp 1）低表达的TM 4-sh细胞为研究对象，探讨了铅的蓄积特性以及ATP依赖的外排泵-多药耐药蛋白1（Mrp 1）在铅排泄中的作用。TM 4细胞具有Mrp样转运活性。mrp 1 mRNA和mrp 1的表达水平在铅处理后先升高后降低。相对mRNA表达量最大差异达10倍。在乙酸铅存在下，TM 4-sh中的累积铅量远高于TM 4。用10-40 μ M醋酸铅处理12小时或24小时后，差异约为2-8倍。转换为无铅培养液后，在1、3、6、9 h时相点，TM 4-sh细胞内铅含量仍高于TM 4细胞（P
Since lead accumulation is toxic to cells, its excretion is crucial for organisms to survive the toxicity. In this study, mouse testis sertoli (TM4) and Mrp1 lower-expression TM4-sh cells were used to explore the lead accumulation characteristics, and the role of ATP-dependent efflux pump-multidrug resistance protein 1 (Mrp1) in lead excretion. TM4 cells possess Mrp-like transport activity. The expression levels of mrp1 mRNA and Mrp1 increased after lead treatments at first and then decreased. The maximum difference of relative mRNA expression reached 10 times. In the presence of lead acetate, the amount of cumulative lead in TM4-sh was much higher than that in TM4. After the treatment with lead acetate at 10-40 mu M for 12 h or 24 h, the differences were about 2-8 times. After with the switch to lead-free medium, the cellular lead content in TM4-sh remains higher than that in TM4 cells at 1,3, 6, and 9h time points (P