Involvement of aquaporin-1 in gastric epithelial cell migration during wound repair
Involvement of aquaporin-1 in gastric epithelial cell migration during wound repair
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DOI：
10.1016/j.bbrc.2009.06.067
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发表时间：
2009-08-28
影响因子：
3.1
通讯作者：
Takeuchi, Koji
中科院分区：
文献类型：
--
作者：
Hayashi, Shusaku;Takahashi, Nobuaki;Takeuchi, Koji
我们检测了大鼠胃上皮细胞系 RGM1 单层中水通道蛋白 (AQP)-1 的表达，并研究了 AQP1 在伤口愈合过程中上皮恢复中的作用。使用旋转硅尖端在汇合细胞单层的中心产生恒定尺寸的圆形伤口。 RGM1 细胞表达 AQP1 mRNA。 Hg2+ (HgCl2) 是 AQP 的抑制剂，以浓度依赖性方式抑制伤口修复过程中的细胞迁移。同样，在 AQP1 被 RNA 干扰敲除的细胞中，细胞迁移也受到损害，导致伤口愈合明显延迟。与对照 RGM1 细胞相比，AQP1 敲除的 RGM1 细胞显示伤口前缘的膜突起（片状伪足）形成减少。这些结果首次表明 AQP1 在伤口愈合过程中胃上皮细胞迁移中发挥着至关重要的作用。 (C) 2009 Elsevier Inc. 保留所有权利。
We examined the expression of aquaporin (AQP)-1 in monolayers of the rat gastric epithelial cell line RGM1 and investigated the roles AQP1 plays in the epithelial restitution during Wound healing. A round Wound of constant size was produced in the center of a confluent cell monolayer using a rotating Silicon tip. The RGM1 cells expressed AQP1 mRNA. Hg2+ (HgCl2), an inhibitor of AQPs, Suppressed cell migration during wound repair in a concentration-dependent manner. Likewise, cell migration was also impaired in cells in which AQP1 was knocked down by RNA interference, resulting in a marked delay Of Wound healing. The AQP1 knockdown RGM1 cells showed a decrease in the formation of membrane protrusions (lamellipodia) at the leading edge of the Wound as compared with control RGM1 cells. These results suggest for the first time that AQP1 plays a crucial role in gastric epithelial cell Migration during Wound healing. (C) 2009 Elsevier Inc. All rights reserved.