Density of Tumor-Associated Macrophages Correlates with Lymph Node Metastasis in Papillary Thyroid Carcinoma
Density of Tumor-Associated Macrophages Correlates with Lymph Node Metastasis in Papillary Thyroid Carcinoma
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DOI：
10.1089/thy.2011.0452
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发表时间：
2012-09-01
期刊：
影响因子：
6.6
通讯作者：
Ning, Guang
中科院分区：
文献类型：
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作者：
Qing, Wei;Fang, Wei-Yuan;Ning, Guang
背景资料：肿瘤相关巨噬细胞（TAM）最近被认为是许多癌症（包括晚期甲状腺癌）肿瘤发生的重要参与者。然而，在甲状腺乳头状癌（PTC），最常见的甲状腺癌的作用，尚未建立。我们假设，TAMs也促进肿瘤进展PTC.Methods：我们调查TAMs密度在甲状腺良性病变和PTC肿瘤的CD 68免疫染色。CD 68阳性细胞密度与PTC患者的临床病理特征进一步相关。最后，TAMs从PTC肿瘤和细胞因子和受体profiling.Results的表型分离：TAMs的整体密度被发现是显着较高的PTC肿瘤，甲状腺肿和滤泡性腺瘤相比。与I/II期相比，TNM（肿瘤-淋巴结-转移）III/VI期TAMs密度与淋巴结转移呈正相关。在其他常见的肿瘤特征中没有观察到相关性，包括BRAF突变。分离出的TAM具有高水平的M2相关细胞因子和受体，使M2成为主要的TAM表型。结论：TAM可能在PTC的进展中发挥功能性作用。
Background: Tumor-associated macrophages (TAMs) have recently been recognized as being important players in the tumoriogenesis of many cancers, including advanced thyroid cancer. However, a role in papillary thyroid carcinoma (PTC), the most prevalent thyroid cancer, has not been established. We hypothesized that TAMs also facilitate tumor progression in PTC.Methods: We investigated TAMs density in both benign thyroid lesions and PTC tumors by CD68 immunostaining. CD68-positive cell density was further associated with the clinicopathological characteristics of PTC patients. Finally, TAMs were isolated from PTC tumors and phenotyped by cytokine and receptor profiling.Results: The overall density of TAMs was found to be significantly higher in PTC tumors, compared with thyroid goiter and follicular adenoma. The density of TAMs was positively associated with lymph node metastasis in TNM (tumor-node-metastasis) stages III/VI compared with stages I/II. No association was observed in other common tumor features, including the BRAF mutation. The isolated TAMs presented with high levels of M2-associated cytokine and receptors, making M2 the predominant TAM phenotype.Conclusions: TAMs may play a functional role in the progression of PTC.