How to make a cardiomyocyte
How to make a cardiomyocyte
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DOI：
10.1242/dev.091538
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发表时间：
2014-12-01
期刊：
影响因子：
4.6
通讯作者：
Chien, Kenneth R.
中科院分区：
文献类型：
--
作者：
Spaeter, Daniela;Hansson, Emil M.;Chien, Kenneth R.
在发育过程中，心脏发生是由一个家庭的心脏祖细胞，建立不同的心脏区域精心策划。每个区域都包含不同的细胞类型，这些细胞聚集形成单个心脏隔室的复杂结构。心肌细胞是在心脏中发现的主要细胞类型，并确保腔室的收缩和整个身体的有效血液流动。由于大量心肌细胞的损失及其再生受损组织的有限内在能力，心肌损伤通常导致心力衰竭，使其成为全球发病率和死亡率的主要原因之一。在这篇文章中，我们讨论了如何洞察心脏发育背后的分子和细胞框架，以指导心肌细胞的体外特化，无论是通过多能干细胞的定向分化还是通过直接谱系转换。另外的策略，以产生心肌细胞原位，如内源性心脏祖细胞的再活化和诱导心肌细胞增殖，也将进行讨论。
During development, cardiogenesis is orchestrated by a family of heart progenitors that build distinct regions of the heart. Each region contains diverse cell types that assemble to form the complex structures of the individual cardiac compartments. Cardiomyocytes are the main cell type found in the heart and ensure contraction of the chambers and efficient blood flow throughout the body. Injury to the cardiac muscle often leads to heart failure due to the loss of a large number of cardiomyocytes and its limited intrinsic capacity to regenerate the damaged tissue, making it one of the leading causes of morbidity and mortality worldwide. In this Primer we discuss how insights into the molecular and cellular framework underlying cardiac development can be used to guide the in vitro specification of cardiomyocytes, whether by directed differentiation of pluripotent stem cells or via direct lineage conversion. Additional strategies to generate cardiomyocytes in situ, such as reactivation of endogenous cardiac progenitors and induction of cardiomyocyte proliferation, will also be discussed.