PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador:: A comparison with classical diagnostic methods
PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador:: A comparison with classical diagnostic methods
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DOI：
10.2307/3285616
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发表时间：
1999-04-01
影响因子：
1.3
通讯作者：
Harris, E
中科院分区：
文献类型：
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作者：
Aviles, H;Belli, A;Harris, E
采用一种简化的聚合酶链式反应(PCR)方法对疑似皮肤利什曼病患者临床标本中的利什曼原虫进行检测和分型。以培养物为参照标准，我们的PCR检测方法比传统的诊断技术更敏感(92%)，包括显微镜(42%)、组织学染色(33%)和IGE酶联免疫吸附试验(20%)。该聚合酶链式反应也是100%特异的。皮损活检和皮损抽吸物培养的寄生虫经聚合酶链式反应鉴定为巴西利什曼原虫。在这项研究中，我们证明了在利什曼病流行的发展中国家，简化的聚合酶链式反应分析方法对引起皮肤利什曼病的寄生虫同时诊断和分型的适用性。
A Simplified polymerase chain reaction (PCR)-based assay was used for detection and typing of Leishmania parasites in clinical specimens from patients suspected of cutaneous leishmaniasis. Using cultures as the reference standard, our PCR detection method was more sensitive (92%) than classical diagnostic techniques, including microscopy (42% sensitivity), histologic staining (33%), and Ige enzyme-linked immunosorbent (20%). The PCR assay was also 100% specific. Parasites in both lesion biopsies and isolates cultured from lesion aspirates were identified as Leishmania braziliensis by PCR. In this study, we have demonstrated the suitability of simplified PCR assays for the simultaneous diagnosis and typing of parasites causing cutaneous leishmaniasis in a developing country where leishmaniasis is endemic.