Regulation of hexose transporters in chicken embryo fibroblasts: Stimulation by the phorbol ester TPA leads to increased numbers of functioning transporters
Regulation of hexose transporters in chicken embryo fibroblasts: Stimulation by the phorbol ester TPA leads to increased numbers of functioning transporters
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鸡胚成纤维细胞中己糖转运蛋白的调节：佛波酯 TPA 的刺激导致功能性转运蛋白数量增加
DOI：
10.1002/jcp.1041270204
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发表时间：
1986
影响因子：
5.6
通讯作者：
K. Isselbacher
中科院分区：
文献类型：
--
作者：
Kazuo Yamada;L. Tillotson;K. Isselbacher
正如在许多类型的培养细胞中观察到的那样，鸡胚成纤维细胞（CEF）暴露于肿瘤启动子12‐O‐十四烷酰磷- 13‐醋酸酯（TPA）时，在4小时内己糖运输活性增加了3‐4倍。尽管[3H]亮氨酸掺入到酸不溶性部分中有20%的适度下降，但运输活性还是增加了。环己亚胺在很大程度上（但不是完全）阻断了TPA暴露期间转运活性的增加。TPA的作用与葡萄糖饥饿诱导的己糖运输活性增强有些相似。此外，TPA对葡萄糖饥饿无加性影响。用经或不经TPA处理的CEF制备了质膜富集组分。与对照CEF制备的膜相比，由TPA暴露的细胞制备的膜具有两倍的立体特异性D -葡萄糖运输活性以及D -葡萄糖抑制[3H]细胞松弛素B结合。当膜暴露于TPA体外时，对转运没有影响。这些结果提供了强有力的证据，表明TPA暴露会导致功能性转运蛋白数量的增加，这种作用主要需要蛋白质合成。
As has been observed with many types of cultured cells, chicken embryo fibroblasts (CEF) when exposed to the tumor promoter 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) develop a 3‐ to 4‐fold increase in hexose transport activity in 4 h. This increase in transport activity occurred despite a modest decline of 20% in [3H]leucine incorporation into acid insoluble fractions. Cycloheximide largely, but not completely, blocked the increase in transport activity during TPA exposure. The effects of TPA were somewhat similar to those of glucose starvation induced enhancement of hexose transport activity. Furthermore, with TPA there was no additive effect to that produced by glucose starvation. Plasma membrane enriched fractions were prepared from CEF treated with or without TPA. Membranes prepared from TPA exposed cells had a two‐fold enhancement of stereospecific D‐glucose transport activity as well as D‐glucose inhibitable [3H]cytochalasin B binding as compared to the membranes from control CEF. There was no effect on transport when membranes were exposed to TPA in vitro. These results provide strong evidence that TPA exposure leads to an increase in the number of functioning transporters, an effect largely requiring protein synthesis.
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脂肪细胞中胰岛素敏感的葡萄糖转运机制的能量依赖和蛋白质合成无关的再循环。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
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DOI：
10.1073/pnas.79.7.2286
发表时间：
1982
影响因子：
11.1
作者：
Pessin,JE;Tillotson,LG;Yamada,K;Gitomer,W;Carter-Su,C;Mora,R;Isselbacher,KJ;Czech,MP
通讯作者：
Czech,MP
DOI：
10.1073/pnas.80.1.36
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
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VANDENBARK, GR
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影响因子：
4.7
作者：
Verma,AK;Bryan,GT;Reznikoff,CA
通讯作者：
Reznikoff,CA