Electro-magnetic Templates with Magnetic Nanoparticles for Cell-based Assays
Electro-magnetic Templates with Magnetic Nanoparticles for Cell-based Assays
复制标题
用于基于细胞的检测的具有磁性纳米颗粒的电磁模板
DOI：
10.1016/j.phpro.2016.05.003
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
A. Khitun
中科院分区：
文献类型：
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作者：
F. Gertz;A. Khitun
我们讨论了一个专门设计的电磁模板与磁性纳米粒子的细胞为基础的测定的可能性。迫切需要一种特殊类型的硬件，允许生物细胞操作。我们开发了一种使用电磁模板和磁性纳米颗粒进行生物细胞操作的原创技术。这种方法的本质是使用载流导线系统产生非均匀磁场分布。磁场的梯度导致纳米颗粒朝向磁能最小值移动。反过来，磁性纳米颗粒的流动将生物细胞拖向同一方向。我们目前的实验数据上的生物细胞（红细胞）操纵磁铁矿（Fe 3 O 4）在专门设计的模板，结果表明控制生物细胞的运动和破坏，通过溶血。这种技术使我们能够捕获和移动位于载流导线附近（10-20微米）的细胞。最有趣的结果之一显示了红细胞在两个导电轮廓之间的周期性运动，其频率由电路控制。所获得的结果证明了可用于基于细胞的测定的细胞操作的可行性。
We discuss the possibility of a specially designed electro-magnetic template with magnetic nanoparticles for cell-based-assays. There is an urgent need for a special type of hardware allowing for biological cell manipulation. We have developed an original technique of using electro-magnetic templates with magnetic nanoparticles for biological cell manipulation. The essence of this approach is to generate a non-uniform magnetic field profile using a system of electric current carrying wires. The gradient of the magnetic field results in the movement of the nanoparticles towards the magnetic energy minima. In turn, the flow of magnetic nanoparticles drags biological cells in the same direction. We present experimental data on biological cells (erythrocytes) manipulations by magnetite (Fe3O4) on specially designed templates The results show controlled biological cell motion and destruction via haemolysis. This technique allows us to capture and to move cells located in the vicinity (10-20 microns) of the current-carrying wires. One of the most interesting results shows a periodic motion of erythrocytes between the two conducting contours, which frequency is controlled by the electric circuit. The obtained results demonstrate the feasibility of cell manipulation which can be utilized in cell-based assays.