Identification and Isolation of Japanese Encephalitis Virus Genotype IV from Culex vishnui Collected in Bali, Indonesia in 2019
Identification and Isolation of Japanese Encephalitis Virus Genotype IV from Culex vishnui Collected in Bali, Indonesia in 2019
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DOI：
10.4269/ajtmh.20-1554
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发表时间：
2021-09-01
影响因子：
3.3
通讯作者：
Sawabe, Kyoko
中科院分区：
文献类型：
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作者：
Faizah, Astri Nur;Kobayashi, Daisuke;Sawabe, Kyoko
日本脑炎病毒（JEV）在猪、候鸟和库蚊之间传播，并且在亚洲本土传播了近世纪。尽管印度尼西亚是JEV多样性最高的国家，但针对印度尼西亚病媒的监测很少。本研究收集了来自印度尼西亚巴厘岛塔巴南Regency几个地点的蚊子。我们捕获了3，032只成年库蚊，并将其分为7种，其中库蚊亚组约占总数的90%。通过Cx的下一代测序（NGS）分析鉴定了日本脑炎病毒。vishnui蚊子池。遗传学和系统发育分析表明，JEV基因型（G）IV。与2017年在巴厘岛从猪血清中获得的其他JEV GIV分离株以及2019年从澳大利亚有巴厘岛旅行史的人类患者中获得的其他JEV GIV分离株的核苷酸同一性为99%。这一发现表明，JEV GIV在限制地区持续存在，并在猪蚊病媒之间传播。
Japanese encephalitis virus (JEV) is transmitted between swine, migratory birds, and Culex mosquitoes, and has circulated indigenously in Asia for almost a century. Despite being the country with the highest JEV diversity, surveillance targeting of Indonesia's vectors is scarce. This study collected mosquitoes from several locations in Tabanan Regency, Bali Island, Indonesia. We captured and classified 3,032 adult Culex mosquitoes into seven species, with Culex vishnui subgroup mosquitoes making up approximately 90% of the total. Japanese encephalitis virus was identified by next-generation sequencing (NGS) analysis of a Cx. vishnui mosquito pool. Genetic and phylogenetic analysis revealed the JEV as genotype (G) IV. The nucleotide identity was 99% with other JEV GIV isolates obtained from swine sera in 2017 on Bali Island and from a human patient in Australia with a travel history to Bali in 2019. This finding indicated that JEV GIV persists in restricted areas and is circulating between swine-mosquito vectors.