Possible role of calcineurin in heating-related increase of rat muscle mass
Possible role of calcineurin in heating-related increase of rat muscle mass
复制标题
DOI：
10.1016/j.bbrc.2005.04.096
复制
发表时间：
2005-06-17
影响因子：
3.1
通讯作者：
Yoshioka, T
中科院分区：
文献类型：
--
作者：
Kobayashi, T;Goto, K;Yoshioka, T
本研究的目的是探讨钙调磷酸酶相关的细胞内信号在热应激相关性肌肉肥大中的作用。纯种;将7周龄的训练雄性大鼠随机分为4组：(1)对照组（CC, n = 15），(2)注射环孢素A （CsA）组（CA, n = 15），(3)热应激组（HC, n = 15），(4)注射环孢素A热应激组（HA, n = 15）。热应激组（HC和HA）在不麻醉的情况下，在受控热室中暴露于41℃的高温下（60名修女）。暴露后1、7和14天分别解剖比目鱼肌和指长伸肌（EDL）并称重。热暴露7 d后比目鱼的干、湿重均增加。热应激后第1天和第7天，两组肌肉中热休克蛋白72 （HSP72）和钙调磷酸酶的表达均升高。钙调磷酸酶的特异性抑制剂CsA抑制热应激引起的肌肉重量增加和钙调磷酸酶的表达；尤其是比目鱼。这些观察结果表明，钙调磷酸酶依赖的信号通路可能在热应激相关的骨骼肌肥大中起重要作用。应用热应力对骨骼肌可能是一个有用的工具，以获得肌肉质量和力量产生不仅在运动员，而且在病人康复期间。(c) 2005爱思唯尔公司版权所有。
The purpose of this study was to investigate the contribution of calcineurin-related intracellular signal for heat-stress-associated muscle hypertrophy. Wistar;train male rats (7-week-old) were randomly divided into four groups: (1) control (CC, n = 15), (2) control with the injection of cyclosporine A (CsA) (CA, n = 15), (3) heat-stressed (HC, n = 15), and (4) heat-stressed with the injection of CsA (HA, n = 15). The heat-stress groups (HC and HA) were exposed to heat (41 degrees C for 60 nun) in a controlled heat chamber without anesthesia. Soleus and extensor digitorum longus (EDL) muscles were dissected and weighed 1, 7, and 14 days after the exposure. Wet and dry weights of soleus were increased 7 days following heat exposure. The expressions of heat shock protein 72 (HSP72) and calcineurin in both muscles were also increased within 1 and 7 days following heat-stress, respectively. Administration of CsA, a specific inhib,tor for calcineurin, depressed heat-stress-associated increase of muscle weight and calcineurin expression; especially in soleus. These observations suggest that a calcineurin-dependent signaling pathway may play an important role in the heat-stress-related skeletal muscular hypertrophy. Application of heat-stress to skeletal muscles may be a useful tool to gain muscular mass and force generation not only in athletes, but also in patients during rehabilitation. (c) 2005 Elsevier Inc. All rights reserved.