The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture onEnterococcus faecalis: The Influence of Different Light Sources
The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture onEnterococcus faecalis: The Influence of Different Light Sources
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DOI：
10.3390/app10124290
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发表时间：
2020-06-01
影响因子：
2.7
通讯作者：
Bordea, Ioana Roxana
中科院分区：
文献类型：
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作者：
Chiniforush, Nasim;Pourhajibagher, Maryam;Bordea, Ioana Roxana
本研究的目的是评价叶绿素-藻蓝蛋白混合物（光活性+）作为光敏剂（PS）在抗菌光动力疗法（aPDT）中对粪肠球菌（E. faecalis）计数的体外影响。粪便）使用不同的光源。使用针对大肠杆菌的微生物细胞活力测定法评估了使用不同光源（包括二极管激光器（λ = 660 nm）、二极管激光器（λ = 635 nm）、LED（λ = 450 +/- 30 nm）单独或组合使用叶绿素-藻蓝蛋白混合物的aPDT的抗菌效果。粪便。此外，通过MTT测定评估光活性+对人牙龈成纤维细胞（HuGu）的细胞毒性;当用红色波长（635 nm，660 nm）以及LED（420-480 nm）和红色波长（635 nm，660 nm）的组合处理时，粪肠球菌生长与对照组相比显著降低（p< 0.05）。与对照组相比，暴露于光活性+的活细胞数量没有显著减少（p< 0.05）。该研究表明，与对照相比，叶绿素-藻蓝蛋白混合物的应用和发射红光的照射实现了更好的细菌计数减少的结果。由于细胞毒性可忽略不计，该组件可安全应用。
The purpose of this study was to evaluate the in vitro effect of the chlorophyllin-phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count ofEnterococcus faecalis(E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin-phycocyanin mixture using different light sources including diode laser (lambda = 660 nm), diode laser (lambda = 635 nm), LED (lambda = 450 +/- 30 nm) alone or in combination was assessed using microbial cell viability assay againstE. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay;E.faecalisgrowth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420-480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p< 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p< 0.05). This study shows that the application of chlorophyllin-phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity.