Pro-inflammatory cytokine release and cell growth inhibition in primary human oral cells after exposure to endodontic sealer
Pro-inflammatory cytokine release and cell growth inhibition in primary human oral cells after exposure to endodontic sealer
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DOI：
10.1111/iej.12230
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发表时间：
2014-09-01
影响因子：
5
通讯作者：
Trubiani, O.
中科院分区：
文献类型：
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作者：
Diomede, F.;Caputi, S.;Trubiani, O.
目的检测单甲基丙烯酸酯基氨基甲酸乙酯二甲基丙烯酸酯（UDMA）（EndoRez）对体外扩增的人牙髓干细胞（hDPSC）、人牙周膜干细胞（hPDLSC）、人牙龈成纤维细胞（hGF）和成骨细胞（hOST）的细胞生长和促炎细胞因子释放的毒性。成骨细胞和成纤维细胞来源于5个年轻供体。在体外分离后，将hDPSC、hPDLSC、hGF和hOST接种到基于树脂的密封剂中24、48、72小时直至1周。分析形态学特征和细胞生长以及促炎性白细胞介素（IL）6、IL 8、IL 12和肿瘤坏死因子（TNF）α的释放。在细胞生长和白细胞介素分泌的差异进行了分析，统计学意义的双向ANOVA检验多重comparison.Results暴露于根管牙髓炎的基础上UDMA导致在24小时的孵育中的生存口腔细胞减少50%。在检查的细胞培养物中不存在明显的形态学变化。在48 h、72 h和1周培养时间后，明显可见进行性细胞生长。结论在体外，EndoRez可与原代人牙髓干细胞、牙周膜干细胞、成纤维细胞和骨基质细胞相互作用，通过抑制细胞生长和上调IL 6、IL 8、IL 12和TNF α等促炎因子，对生物系统造成损伤。
Aim To assay the toxicity of the single-methacrylate-based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro-inflammatory cytokines release, in expanded ex vivo human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPDLSCs), human gingival fibroblasts (hGFs) and human osteoblasts (hOSTs).Methodology Dental pulp and periodontal ligament stem cells, osteoblasts and fibroblasts were derived from five young donors. After in vitro isolation, hDPSCs, hPDLSCs, hGFs and hOSTs were seeded to resin-based sealers for 24, 48, 72 h up to 1 week. The morphological features and the cell growth and the release of pro-inflammatory interleukin (IL) 6, IL8, IL12 and tumour necrosis factor (TNF) alpha were analysed. Differences in cell growth and in interleukin secretion were analysed for statistical significance with two-way ANOVA tests for multiple comparisons.Results Exposure to endodontic sealer based on UDMA resulted in a 50% decrease in survival oral cells at 24 h of incubation. No evident morphological changes were present in cell cultures examined. After 48 h, 72 h and 1-week culture time, a progressive cell growth was evident. A significant up-regulation of IL6, IL8, IL12 and TNF alpha cytokines in cells in contact with the dental sealer compared to the control was observed.Conclusion In vitro, EndoRez interacted with primary human hDPSCs, hPDLSCs, hGFs and hOSTs causing damage to biological system evidenced through cell growth inhibition and up-regulation of IL6, IL8, IL12 and TNF alpha proinflammatory mediators.