Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge.
Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge.
复制标题
灿烂弧菌攻击后海参体腔细胞免疫途径相关基因的转录组分析和发现
DOI：
10.3390/ijms160716347
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发表时间：
2015-07-17
影响因子：
5.6
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Gao Q;Liao M;Wang Y;Li B;Zhang Z;Rong X;Chen G;Wang L
灿烂弧菌（Vibrio splendidus）是引起海参（Apostichopus sputum）皮肤溃疡综合征的主要致病因子之一，严重限制了海参养殖业的发展。为筛选参与海参抗弧菌攻击的免疫相关基因，并探讨其分子机制，收集了海参抗弧菌攻击和敏感生物型的相关转录组和基因表达谱进行分析。获得总共319，455，942个修剪的读段，将其组装成186，658个重叠群。之后，将89，891个代表性重叠群（没有同种型）聚类。基因表达谱分析表明，与对照组相比，细菌耐药组有358个差异表达基因（DEG），细菌敏感组有102个差异表达基因。根据已发表的文献和BLAST、GO、KEGG的注释信息，从DEG中鉴定出30个细菌抗性基因和19个细菌敏感基因。qRT-PCR结果与RNA-Seq结果一致。此外，许多DGE还参与了免疫信号相关通路，如内吞、溶酶体、MAPK、趋化因子和ERBB信号通路。
Vibrio splendidus is identified as one of the major pathogenic factors for the skin ulceration syndrome in sea cucumber (Apostichopus japonicus), which has vastly limited the development of the sea cucumber culture industry. In order to screen the immune genes involving Vibrio splendidus challenge in sea cucumber and explore the molecular mechanism of this process, the related transcriptome and gene expression profiling of resistant and susceptible biotypes of sea cucumber with Vibrio splendidus challenge were collected for analysis. A total of 319,455,942 trimmed reads were obtained, which were assembled into 186,658 contigs. After that, 89,891 representative contigs (without isoform) were clustered. The analysis of the gene expression profiling identified 358 differentially expression genes (DEGs) in the bacterial-resistant group, and 102 DEGs in the bacterial-susceptible group, compared with that in control group. According to the reported references and annotation information from BLAST, GO and KEGG, 30 putative bacterial-resistant genes and 19 putative bacterial-susceptible genes were identified from DEGs. The qRT-PCR results were consistent with the RNA-Seq results. Furthermore, many DGEs were involved in immune signaling related pathways, such as Endocytosis, Lysosome, MAPK, Chemokine and the ERBB signaling pathway.