Melting curve analysis in a snapshot
Melting curve analysis in a snapshot
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DOI：
10.1063/1.2790806
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发表时间：
2007-09-24
影响因子：
4
通讯作者：
Braun, Dieter
中科院分区：
文献类型：
--
作者：
Baaske, Philipp;Duhr, Stefan;Braun, Dieter
分子的热变性是生物化学和诊断中必不可少的方法，包括单核苷酸多态性的测量和蛋白质的结合分析。提出了一种全光高速测量熔化曲线的方法。用红外激光局部加热薄片水，得到20到100摄氏度之间的空间温度分布。用荧光显微镜记录50毫秒内的熔化曲线。这比最先进的荧光测定法快10000倍，并且对DNA发夹的验证示例产生相同的结果。(c) 2007年美国物理学会。
The thermal denaturation of molecules is an essential method in biochemistry and diagnostics, including the measurement of single nucleotide polymorphisms and the binding analysis of proteins. We present a method for the all-optical high speed measurement of melting curves. A thin sheet of water is locally heated with an infrared laser to obtain a spatial temperature distribution between 20 and 100 degrees C. Using a fluorescence microscope a melting curve is recorded within 50 ms. This is about 10 000-times faster than state-of-the-art fluorometry and yields the same results for the validation example of a DNA hairpin. (c) 2007 American Institute of Physics.