A SIMPLE IN-VITRO MODEL FOR GROWTH-CONTROL OF BACTERIAL BIOFILMS
A SIMPLE IN-VITRO MODEL FOR GROWTH-CONTROL OF BACTERIAL BIOFILMS
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DOI：
10.1111/j.1365-2672.1995.tb03128.x
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发表时间：
1995-07-01
期刊：
影响因子：
--
通讯作者：
GILBERT, P
中科院分区：
文献类型：
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作者：
HODGSON, AE;NELSON, SM;GILBERT, P
发现灌注式生物膜发酵罐不适合于金黄色葡萄球菌和铜绿假单胞菌生物膜的长期培养和生长速率控制。在一种简化的方法中，这些生物体的生物膜在用培养基灌注的Sorbarod滤塞内生长。建立了假稳态，其在几天内是稳定的，在该假稳态下，生物膜的生长速率是可再现的、可测量的并且显著慢于肉汤培养物。环境扫描电子显微镜的解剖山梨糖醇表现出协会的细胞与个别纤维素纤维的表面，和生长特性的生物膜。从Sorbarod模型中回收的相对较高的细胞数促进了生物膜群体和自发释放的细胞的生物化学调查。SDS-PAGE显示生物膜和洗脱群体的蛋白质谱之间存在显着差异，其中包括葡萄球菌。金黄色葡萄球菌，抑制48 kDa的蛋白质和增加的表达的21 kDa的蛋白质相对于在相同的生长速率培养的增殖控制。本文证明了该方法对于适合于生化分析的生物膜样品的培养的适用性。
The perfused biofilm fermenter was found to be unsuitable for the long-term culture and growth rate control of Staphylococcus aureus and Pseudomonas aeruginosa biofilms. In a simplified approach, biofilms of these organisms were grown within Sorbarod filter plugs which were perfused with culture medium. Pseudo-steady states were established which were stable over several days at which the growth rate of the biofilm was reproducible, measurable and significantly slower than in broth culture. Environmental scanning electron microscopy of dissected Sorbarods demonstrated an association of cells with the surfaces of individual cellulose fibres, and growth characteristic of biofilms. Relatively high cell numbers recovered from the Sorbarod model facilitated biochemical investigations of biofilm populations and cells released spontaneously from them.SDS-PAGE demonstrated significant differences between the protein profiles of biofilm and eluted populations, which include, in Staph. aureus, the repression of a 48 kDa protein and increased expression of a 21 kDa protein relative to planktonic controls cultured at equivalent growth rates. The paper demonstrates the suitability of the approach for the culture of biofilm samples which are suitable for biochemical analysis.