Quantitative evaluation of adhesion of lactobacilli isolated from human intestinal tissues to human colonic mucin using surface plasmon resonance (BIACORE assay)
Quantitative evaluation of adhesion of lactobacilli isolated from human intestinal tissues to human colonic mucin using surface plasmon resonance (BIACORE assay)
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DOI：
10.1111/j.1365-2672.2006.03061.x
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发表时间：
2007-01-01
影响因子：
4
通讯作者：
Saito, T.
中科院分区：
文献类型：
--
作者：
Kinoshita, H.;Uchida, H.;Saito, T.
目的：从人肠道粘液层中分离乳酸杆菌，并采用BIACORE法评价其粘附能力。方法与结果：采用常规平板培养法从正常人肠道组织粘液层中分离出30株乳酸杆菌。通过 16S rDNA 序列与数据库的同源性比较，将菌株鉴定为唾液乳杆菌 (26%)、发酵乳杆菌 (13%)、加氏乳杆菌 (10%)、副干酪乳杆菌 (7%)、干酪乳杆菌 (3%)、粘膜乳杆菌 (3%) 和植物乳杆菌 (3%)。使用 BIACORE 测定，植物乳杆菌 LA 318 在 115 30 +/- 12 37 共振单位 (RU) 下显示出对人结肠粘蛋白 (HCM) 的最高粘附力。 LA 318 菌株细胞壁表面蛋白对 HCM 的粘附显着高于对牛血清白蛋白的粘附（BSA；P < 0.05）。结论：我们分离了 30 株乳酸杆菌。唾液乳杆菌是本研究中分离的乳杆菌的主要种类。显示了从人横结肠分离的菌株LA 318与其粘蛋白的粘附性。这种粘附可能是由细菌细胞表面的凝集素样成分介导的。该研究的意义和影响：这是第一项从人体肠道组织中分离出乳酸杆菌并显示其粘附于 HCM 的研究。
Aims: To isolate lactobacilli from the mucus layer of the human intestine and evaluate their adhesion abilities using a BIACORE assay.Methods and Results: Thirty strains of lactobacilli were isolated from the mucus layer of normal human intestinal tissues using conventional plate culture. The strains were identified using homology comparisons of the 16S rDNA sequence to databases as Lactobacillus salivarius (26%), Lactobacillus fermentum (13%), Lactobacillus gasseri (10%), Lactobacillus paracasei (7%), Lactobacillus casei (3%), Lactobacillus mucosae (3%) and Lactobacillus plantarum (3%). Lactobacillus plantarum LA 318 shows the highest adhesion to human colonic mucin (HCM) using the BIACORE assay at 115 30 +/- 12 37 resonance unit (RU). The adhesion of cell wall surface proteins from strain LA 318 was significantly higher to HCM than to bovine serum albumin (BSA; P < 0.05).Conclusions: We isolated 30 strains of lactobacilli. Lactobacillus salivarius was the predominant species of lactobacilli isolated in this study. The adhesion of strain LA 318 isolated from human transverse colon to its mucin was shown. The adhesion could be mediated by lectin-like components on the bacterial cell surface.Significance and Impact of the Study: This is the first study where lactobacilli were isolated from human intestinal tissues and shown to adhere to HCM.