Molecular characterization and phylogenetic analyses of Fasciola gigantica of buffaloes and goats in Punjab, Pakistan
Molecular characterization and phylogenetic analyses of Fasciola gigantica of buffaloes and goats in Punjab, Pakistan
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DOI：
10.1016/j.parint.2021.102288
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发表时间：
2021-01-30
影响因子：
1.9
通讯作者：
Ichikawa-Seki, Madoka
中科院分区：
文献类型：
--
作者：
Rehman, Zia Ur;Tashibu, Atsushi;Ichikawa-Seki, Madoka
在印度次大陆，片形吸虫被认为是引起片形吸虫病的主要病原体，在畜牧业中导致数百万美元的生产损失。了解了该虫的传播来源和传播方式。南极洲很重要。2017年至2018年在巴基斯坦旁遮普从水牛和山羊身上采集的共计53只片形吸虫，经鉴定为F.基于磷酸烯醇式丙酮酸羧激酶（pepck）的多重PCR和DNA聚合酶δ（pold）的PCR-限制性片段长度多态性（RFLP），F.通过对线粒体标记、NADH脱氢酶亚基1（nad 1）和细胞色素C氧化酶亚基1（cox 1）的遗传分析，表明水牛和山羊的嗜热链球菌为嗜热链球菌。对17种nad 1单倍型进行系统发育分析。与印度次大陆邻近国家的拟南芥的比较表明，在巴基斯坦鉴定的所有单倍型都聚在单倍群A中。具有8种单倍型的片形吸虫与印度单倍型有亲缘关系，可能沿着家畜的迁徙，从印度起源向巴基斯坦扩展。与此相反，其余9个单倍型与任何邻国都不共享，表明独立起源，可能来自邻近的中东国家。然而，由于本研究的样本量非常有限，因此需要谨慎解释。我们的研究提供了一种可用于调查F.南极洲
Fasciola gigantica is considered to be a major pathogen causing fasciolosis in the Indian subcontinent, resulting in production losses of millions of dollars in the livestock industry. Understading the dispersal origin and the patterns of spread of F. gigantica is important. A total of 53 Fasciola flukes collected from buffaloes and goats in Punjab, Pakistan between 2017 and 2018 were identified as F. gigantica based on the multiplex PCR for the phosphoenolpyruvate carboxykinase (pepck) and the PCR-restriction fragment length polymorphism (RFLP) for DNA polymerase delta (pold). A significant genetic difference between F. gigantica from buffaloes and goats was indicated by the genetic analyses of mitochondrial markers, NADH dehydrogenase subunit 1 (nad1) and cyto-chrome C oxidase subunit 1 (cox1). Phylogenetic analysis of the seventeen nad1 haplotypes of F. gigantica from Pakistan with those in neighbouring countries of the Indian subcontinent revealed that all the haplotypes identified in Pakistan were clustered in haplogroup A. fasciola gigantica with the eight haplotypes might be expanded in Pakistan from Indian origin, along with the migration of the domestic animals, since they were related to Indian haplotypes. In contrast, the remaining nine haplotypes were not shared with any neighbouring countries, suggesting independent origin, probably from neighbouring Middle East countries. However, cautious interpretation is required due to the very limited samples size of this study. Our study provides a proof of concept for a method that could be used to investigate the epidemiology of F. gigantica.