Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa infection
Drosophila melanogaster as a model host for studying Pseudomonas aeruginosa infection
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DOI：
10.1038/nprot.2009.124
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发表时间：
2009-01-01
期刊：
影响因子：
14.8
通讯作者：
Rahme, Laurence G.
中科院分区：
文献类型：
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作者：
Apidianakis, Yiorgos;Rahme, Laurence G.
果蝇和哺乳动物之间宿主信号通路和组织生理学的保守性允许在果蝇中建立人类宿主与病原体相互作用的模型。在这里，我们提出使用细菌发病机制的遗传易处理的果蝇模型来研究人类机会病原体铜绿假单胞菌的感染。我们描述并比较了两种常用的铜绿假单胞菌感染果蝇的方案：针刺法和注射泵法。每个模型都与检查宿主防御和毒力中的宿主成分和细菌因素相关。可以评估宿主果蝇内的果蝇存活和细菌增殖，作为宿主易感性和病原体毒力潜力的衡量标准。可以确定宿主对铜绿假单胞菌毒力和非毒力菌株的反应概况，从而能够鉴定可能有利于或限制感染的起始和进展的相互作用特异性基因。本文提出的两种方案均可适用于其他微生物病原体的接种和研究。铜绿假单胞菌细胞制备需要24小时，果蝇接种1小时，果蝇存活和细菌增殖1-4天。
Conservation of host signaling pathways and tissue physiology between Drosophila melanogaster and mammals allows for the modeling of human host-pathogen interactions in Drosophila. Here we present the use of genetically tractable Drosophila models of bacterial pathogenesis to study infection with the human opportunistic pathogen Pseudomonas aeruginosa. We describe and compare two protocols commonly used to infect Drosophila with P. aeruginosa: needle-pricking and injector-pumping. Each model has relevance for examining host components and bacterial factors in host defense and virulence. Fly survival and bacterial proliferation within host flies can be assessed as a measure of host susceptibility and pathogen virulence potential. The profiles of host responses toward P. aeruginosa virulent and non-virulent strains can be determined, enabling the identification of interaction-specific genes that could potentially favor or limit the initiation and progression of infection. Both of the protocols presented herein may be adapted for the inoculation and study of other microbial pathogens. P. aeruginosa cell preparation requires 24 h, fly inoculation 1 h, and fly survival and bacterial proliferation 1-4 d.