Transient silencing of VvCSN5 enhances powdery mildew resistance in grapevine (Vitis vinifera)
Transient silencing of VvCSN5 enhances powdery mildew resistance in grapevine (Vitis vinifera)
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DOI：
10.1007/s11240-021-02098-z
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发表时间：
2021-05-06
影响因子：
3
通讯作者：
Wen, Ying-Qiang
中科院分区：
文献类型：
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作者：
Cui, Kai-Cheng;Liu, Min;Wen, Ying-Qiang
葡萄藤（葡萄属vinifera）是世界上最重要的经济水果作物之一，其产量受到包括由葡萄白粉菌（Erysiphe necator）引起的白粉病在内的各种病原体的严重损失。相比之下，几种野生中国葡萄藤，包括葡萄属伪网状葡萄种百合-35-1，对白粉病病原体具有高度抗性。在此，我们鉴定了一个葡萄藤基因CSN 5（COP 9信号体复合物亚基5），命名为VvCSN 5，该基因在白粉病分离物白粉菌NAFU 1感染期间在抗性'百合-35-1'和感病'汤普森无核'之间差异表达。此外，VvCSN 5在'Thompson Seedless'叶片中的瞬时沉默增强了对En NAFU 1的抗性。这种抗性表现在试图感染部位的细胞壁胼胝质沉积和穿透的表皮细胞的过敏反应样细胞死亡。几个防御相关的标记基因（VvPR 1，VvPR 3，VvPAD 4和VvRBOHD）在VvCSN 5沉默的叶片中具有较高的基础表达水平。此外，我们还发现‘汤普森无核’和‘百合35-1’中CSN 5启动子的结构和活性存在差异，这可能是它们对白粉病抗性不同的原因。综上所述，这些结果表明葡萄藤CSN 5在葡萄对白粉病的应答中起着重要的作用。关键信息VvCSN 5在葡萄属葡萄叶片中的瞬时沉默增强了葡萄对白粉病的抗性。此外，CSN 5启动子在敏感和抗性葡萄中是不同的。
As one of the most economically important fruit crops in the world, the grapevine (Vitis vinifera) suffers significant yield losses from various pathogens including powdery mildew caused by Erysiphe necator. In contrast, several wild Chinese grapevines, including Vitis pseudoreticulata accession Baihe-35-1, are highly resistant to powdery mildew pathogens. Here, we identified a grapevine gene CSN5 (COP9 signalosome complex subunit 5), designated VvCSN5, that was differentially expressed between the resistant 'Baihe-35-1' and susceptible 'Thompson Seedless' during powdery mildew isolate Erysiphe necator NAFU1 infection. Moreover, transient silencing of VvCSN5 in 'Thompson Seedless' leaves enhanced resistance to En NAFU1. This resistance manifested in cell wall callose deposition at attempted infection sites and hypersensitive response-like cell death of penetrated epidermal cells. Several defense-related marker genes (VvPR1, VvPR3, VvPAD4, and VvRBOHD) had higher basal expression levels in VvCSN5-silenced leaves. In addition, we found the structure and activity of CSN5 promoters in 'Thompson Seedless' and 'Baihe-35-1' were different, which may have been behind their different resistances to powdery mildew infection. Taken together, these results implied that grapevine CSN5 plays an important role in the response to powdery mildew infection.Key message Transient silencing of VvCSN5 in Vitis vinifera leaves enhances powdery mildew resistance. In addition, the CSN5 promoters in susceptible and resistant grapevines are different.