Imaging the thermodynamic state of lipid membranes with multiplex CARS microscopy
Imaging the thermodynamic state of lipid membranes with multiplex CARS microscopy
复制标题
利用多重 CARS 显微镜成像脂质膜的热力学状态
DOI：
10.1021/jp014012y
复制
发表时间：
2002-04-11
影响因子：
3.3
通讯作者：
Schins, JM
中科院分区：
文献类型：
--
作者：
Müller, M;Schins, JM
首次展示了多重相干反斯托克斯拉曼散射(CARS)显微成像，并用于显示脂膜的热力学状态，无论是液晶相还是凝胶相。虽然在自发拉曼散射中，低散射截面通常是高分辨率显微成像的禁区，但多路CARS中的信号增强超过4个数量级，能够以真实的帧速率进行显微成像(类似Tomin(-1))。由于同时检测CARS信号的宽光谱窗口，可以直接完成对脂膜热力学状态的识别，而无需对激光器进行任何额外的调谐。我们证明，实验的CARS光谱数据与自发拉曼散射的光谱数据高度一致，其信噪比仅由泊松噪声决定。此外，固有的强度归一化使多路复用汽车成为一种稳健的成像技术。
Multiplex coherent anti-Stokes Raman scattering (CARS) microscopic imaging is demonstrated for the first time and used to visualize the thermodynamic state, either liquid crystalline or gel phase, of lipid membranes. Whereas in spontaneous Raman scattering the low scattering cross section is generally prohibitive for high-resolution microscopic imaging, the signal enhancement in multiplex CARS, by more than 4 orders of magnitude, enables microscopic imaging with realistic frame rates (similar tomin(-1)). Because of the broad spectral window over which the CARS signal is detected simultaneously, the identification of the thermodynamic state of the lipid membranes can be accomplished directly, without any additional tuning of the lasers. We demonstrate that the experimental CARS spectral data are highly consistent with those obtained with spontaneous Raman scattering, with a signal-to-noise ratio determined only by Poisson noise. In addition, the inherent intensity normalization renders multiplex CARS a robust imaging technique.