RECEPTOR AND LIGAND DOMAINS FOR INVASION OF ERYTHROCYTES BY PLASMODIUM-FALCIPARUM
RECEPTOR AND LIGAND DOMAINS FOR INVASION OF ERYTHROCYTES BY PLASMODIUM-FALCIPARUM
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DOI：
10.1126/science.8009226
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发表时间：
1994-06-24
期刊：
影响因子：
56.9
通讯作者：
MILLER, LH
中科院分区：
文献类型：
--
作者：
SIM, BKL;CHITNIS, CE;MILLER, LH
恶性疟原虫的一种175千道尔顿的红细胞结合蛋白EBA-175介导红细胞的侵入。EBA-175的红细胞受体依赖于唾液酸。使用COS细胞上表达的EBA-175的截短部分，将结合红细胞的EBA-175的结构域鉴定为区域II。含有富含半胱氨酸基序的区域II和天然EBA-175特异性结合红细胞膜上的血型糖蛋白A，但不结合血型糖蛋白B。EBA-175的红细胞识别需要唾液酸和血型糖蛋白A的肽骨架。受体和配体结构域的鉴定可能为受体阻断剂和疫苗的合理设计提供建议。
A 175-kilodalton erythrocyte binding protein, EBA-175, of the parasite Plasmodium falciparum mediates the invasion of erythrocytes. The erythrocyte receptor for EBA-175 is dependent on sialic acid. The domain of EBA-175 that binds erythrocytes was identified as region II with the use of truncated portions of EBA-175 expressed on COS cells. Region II, which contains a cysteine-rich motif, and native EBA-175 bind specifically to glycophorin A, but not to glycophorin B, on the erythrocyte membrane. Erythrocyte recognition of EBA-175 requires both sialic acid and the peptide backbone of glycophorin A. The identification of both the receptor and ligand domains may suggest rational designs for receptor blockade and vaccines.