Arabidopsis WIH1 and WIH2 Genes Act in the Transition from Somatic to Reproductive Cell Fate
Arabidopsis WIH1 and WIH2 Genes Act in the Transition from Somatic to Reproductive Cell Fate
复制标题
DOI：
10.1016/j.cub.2011.05.015
复制
发表时间：
2011-06-21
期刊：
影响因子：
9.2
通讯作者：
Laux, Thomas
中科院分区：
文献类型：
--
作者：
Lieber, Diana;Lora, Jorge;Laux, Thomas
背景资料：与动物不同，高等植物在胚胎发育中不建立生殖系，而是在其生命周期的后期从二倍体体细胞形成单倍体生殖细胞。然而，尽管其首要的重要性，很少有人知道这个过渡是如何regulated.Results：在这里，我们表明，WUSCHEL（WUS）基因，最初确定为一个干细胞调节器在拍摄分生组织，需要大孢子发生，从而最终形成女性生殖细胞。WUS通过间接激活WINDHOSE 1（WIH 1）和WIH 2基因的表达在这一过程中发挥作用，这些基因编码在植物和真菌中发现的小肽，但在动物中不存在。WIH基因的功能与四跨膜蛋白型蛋白TORNADO 2（TRN 2）/EKEKO在促进megasporogenesis.Conclusions：总之，我们的研究确定了一个途径，促进生殖细胞形成的体细胞前体细胞。
Background: Unlike animals, higher plants do not establish a germ line in embryo development but form haploid germ cells from diploid somatic cells late in their life cycle. However, despite its prime importance, little is known about how this transition is regulated.Results: Here, we show that the WUSCHEL (WUS) gene, initially identified as a stem cell regulator in the shoot meristem, is required for megasporogenesis and thus ultimately for the formation of female generative cells. WUS functions in this process by indirectly activating the expression of the WINDHOSE1 (WIH1) and WIH2 genes that encode small peptides found in plants and fungi, but not in animals. WIH genes function together with the tetraspanin-type protein TORNADO2 (TRN2)/EKEKO in promoting megasporogenesis.Conclusions: Together, our studies identify a pathway promoting germ cell formation from somatic precursor cells.