Ethanol increases apolipoprotein B mRNA editing in rat primary hepatocytes and McArdle cells.
Ethanol increases apolipoprotein B mRNA editing in rat primary hepatocytes and McArdle cells.
复制标题
DOI：
10.1006/bbrc.1998.9647
复制
发表时间：
1998-11
影响因子：
3.1
通讯作者：
D. Van Mater;M. Sowden;J. Cianci;J. Sparks;C. Sparks;N. Ballatori;H. C. Smith
中科院分区：
文献类型：
--
作者：
D. Van Mater;M. Sowden;J. Cianci;J. Sparks;C. Sparks;N. Ballatori;H. C. Smith
载脂蛋白B（apoB）mRNA编辑涉及由胞苷脱氨酶APOBEC-1催化的位点特异性胞苷至尿苷的转变，其在含有多蛋白质的编辑体的背景下并受其调节。体内ApoB mRNA编辑受组织特异性、发育和代谢调节的影响。我们首次证明，在大鼠原代肝细胞中编辑的apoB mRNA的量显着增加，随后在体外用乙醇瞬时处理。编辑效率的明显变化是剂量依赖性的（从0.1%-2.4%的初始乙醇剂量），并迅速发生。在大鼠肝癌细胞系McArdle RH 7777细胞和稳定的过表达APOBEC-1的McArdle细胞系中，通过2.5%乙醇瞬时处理，编辑的apoB mRNA的比例也显著增加。相比之下，apoB mRNA编辑在人肝癌细胞系，HepG 2细胞和稳定的HepG 2细胞系过表达APOBEC-1没有响应乙醇处理。数据支持编辑活性是乙醇响应性的可能性，但表明这种变化是细胞类型特异性的。
Apolipoprotein B (apoB) mRNA editing involves a site-specific cytidine to uridine transition catalyzed by the cytidine deaminase, APOBEC-1, in the context of and regulated by a multi-protein-containing editosome. ApoB mRNA editing in vivo is subject to tissue specific, developmental and metabolic regulation. We demonstrate for the first time that the amount of edited apoB mRNA in rat primary hepatocytes is markedly increased subsequent to transient treatment with ethanol in vitro. The apparent change in editing efficiency was dose-dependent (from 0.1%-2.4% initial ethanol dose) and occurred with rapid onset. The proportion of edited apoB mRNA was also markedly enhanced in a rat hepatoma cell line, McArdle RH7777 cells and in a stable McArdle cell line over-expressing APOBEC-1 by transient treatment with 2.5 % ethanol. In contrast, the apoB mRNA editing in a human hepatoma cell line, HepG2 cells and a stable HepG2 cell line over-expressing APOBEC-1 did not respond to ethanol treatment. The data support the possibility that editing activity is ethanol-responsive but suggest that this change is cell type-specific.