Differential expression of the L-plastin gene in human colorectal cancer progression and metastasis
Differential expression of the L-plastin gene in human colorectal cancer progression and metastasis
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DOI：
10.1006/bbrc.2001.6047
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发表时间：
2001-12-14
影响因子：
3.1
通讯作者：
Seki, N
中科院分区：
文献类型：
--
作者：
Otsuka, M;Kato, M;Seki, N
为了确定结直肠癌进展中的分子改变，我们分析了来自单个患者的原发性和转移性肿瘤的结肠癌细胞系的基因表达谱。使用我们的内部微阵列研究的 2280 个 cDNA 中，与转移性肿瘤来源的细胞相比，6 个基因（肿瘤相关抗原 L6、L-plastin、酵母核糖体蛋白 S28 的人类同源物、B 细胞易位基因、线粒体天冬氨酸转氨酶和 HLA-A）的表达增加，而 2 个基因（角蛋白 5 和磷酸葡萄糖变位酶）的表达减少原代肿瘤来源的细胞。在这些基因中，我们使用由 58 个临床分层结直肠癌样本组成的组织微阵列在蛋白质水平上评估了 L-plastin 基因（一种肌动蛋白捆绑蛋白）。与我们的微阵列结果一致，L-plastin的表达与癌症分期的进展显着相关。因此，我们的结果表明 L-plastin 基因是一个潜在的转移标记。此外，如此处所示，结合 cDNA 微阵列和组织阵列被认为有助于快速表征候选生物标志物。 (C) 2001 年爱思唯尔科学。
To identify molecular alterations in the progression of colorectal carcinoma, we analyzed gene expression profiles of colon cancer cell lines derived from primary and metastatic tumors from a single patient. Of 2280 cDNAs investigated using our in-house microarray, the expression of 6 genes (tumor-associated antigen L6, L-plastin, the human homologue of yeast ribosomal protein S28, the B-cell translocation gene, mitochondrial aspartate-aminotransferase, and HLA-A) increased, while that of 2 genes (keratin 5 and phosphoglucomutase) decreased in metastatic-tumor-derived cells compared with primary-tumor-derived cells. Of these genes, we assessed the L-plastin gene, an actin-bundling protein, at the protein level using a tissue microarray consisting of 58 clinically stratified colorectal cancer specimens. Consistent with our microarray results, the expression of L-plastin was significantly correlated with the progression of cancer staging. Therefore, our results suggest that the L-plastin gene is a potential metastatic marker. In addition, combining cDNA microarrays and tissue arrays, as shown here, is thought to facilitate the rapid characterization of candidate biomarkers. (C) 2001 Elsevier Science.