Expression of Musashi-1 in human normal colon crypt cells - A possible stem cell marker of human colon epithelium
Expression of Musashi-1 in human normal colon crypt cells - A possible stem cell marker of human colon epithelium
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DOI：
10.1023/a:1024763723240
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发表时间：
2003-08-01
影响因子：
3.1
通讯作者：
Mitsufuji, S
中科院分区：
文献类型：
--
作者：
Nishimura, S;Wakabayashi, N;Mitsufuji, S
Musashi是一种RNA结合蛋白，被认为参与果蝇神经发育过程中的不对称分裂。为了分析哺乳动物Musashi同源物Musashi-1在人类正常结肠隐窝中的表达模式，对从正常结肠粘膜活检标本中分离的155个结肠隐窝进行了评价。将标本固定，显微解剖以分离一些隐窝，用抗Musashi-1抗体（14 H1）免疫染色，并在共聚焦激光扫描显微镜下检查。每个隐窝中Musashi-1阳性细胞的数量为19.0 +/- 7.53（平均值+/- SD）。大多数Musashi-1阳性细胞位于隐窝基部，细胞位置1和10之间。Musashi-1阳性细胞的分布与干细胞的分布相对应，如先前的报道所述，这意味着Musashi-1是结肠隐窝内不对称分裂的关键控制因素。这是首次报道Musashi-1在人结肠隐窝细胞中的表达。
Musashi has been identified as an RNA-binding protein thought to be involved in asymmetric divisions during Drosophila neural development. To analyze expression patterns of mammalian Musashi homolog Musashi-1 in human normal colon crypt, 155 colon crypts separated from biopsy specimens of normal colonic mucosa were evaluated. Specimens were fixed, microdissected to isolate a few crypts, immunostained with anti-Musashi-1 antibody (14H1), and examined under confocal laser scan microscopy. The number of Musashi-1-positive cells in each crypt was 19.0 +/- 7.53 ( mean +/- SD). Most Musashi-1 positive cells were located at the crypt base, between cell positions 1 and 10. Distribution of Musashi-1-positive cells corresponded with that of stem cells, as outlined in previous reports, implying that Musashi-1 is a key control element of asymmetrical division within the colon crypt. This is the first report outlining expression of Musashi-1 in human colon crypt cells.