Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling
Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling
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DOI：
10.1126/science.1080405
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发表时间：
2003-04-25
期刊：
影响因子：
56.9
通讯作者：
Karplus, PA
中科院分区：
文献类型：
--
作者：
Wood, ZA;Poole, LB;Karplus, PA
真核生物2-Cys过氧化物酶（2-Cys Prxs）不仅具有抗氧化作用，而且还能调节过氧化氢介导的信号转导。我们发现，细菌2-Cys Prxs是氧化失活的敏感性要低得多，比真核2-Cys Prxs。通过确定两个序列图案独特的敏感2-Cys Prx和比较的晶体结构的细菌2-Cys Prx在2.2埃分辨率与其他Prx结构，我们定义的敏感性的结构起源。我们认为这种适应允许2-Cys Prxs作为闸门，保持过氧化氢的静息水平低，同时允许在信号转导过程中更高的水平。
Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motifs unique to the sensitive 2-Cys Prxs and comparing the crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with other Prx structures, we define the structural origins of sensitivity. We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction.