Ion-specificities of sulfite and phosphate ion enzyme sensors.
Ion-specificities of sulfite and phosphate ion enzyme sensors.
复制标题
亚硫酸盐和磷酸盐离子酶传感器的离子特异性。
DOI：
10.2116/bunsekikagaku.42.6_397
复制
发表时间：
1993
期刊：
影响因子：
0.2
通讯作者：
T. Wasa
中科院分区：
文献类型：
--
作者：
T. Yao;M. Wada;T. Wasa
采用戊二醛在3-氨基丙基三甲氧基硅烷化的铂盘电极平端交联亚硫酸盐氧化酶与牛血清白蛋白，制备了亚硫酸盐离子酶传感器。同样，将嘌呤核苷磷酸化酶和黄嘌呤氧化酶共固定在玻碳圆盘电极的平端，制成磷酸离子酶传感器。基于酶促反应生成的过氧化氢的电流检测（0.6 V vs. Ag/AgCl），建立了亚硫酸盐传感器；电流响应与亚硫酸盐浓度在5×10-75×10-4 m范围内呈线性关系，该传感器对亚硫酸盐具有高度选择性，但对亚硝酸盐、硫氰酸盐和硫代硫酸盐的响应较小。当肌苷（5mm）加入含有磷酸盐的0.1 M硼酸盐缓冲溶液（pH 7.5）时，磷酸盐传感器给出响应。该传感器是基于安培检测（0.5 V vs. Ag/AgCl）尿酸产生两个连续的酶促反应。在37±0.2°C下，电流响应与磷酸盐浓度在5×10-72×10-5 M范围内呈线性关系。这个传感器基本上是针对磷酸盐的。
A sulfite ion enzyme sensor was prepared by cross-linking sulfite oxidase with bovine serum albumin using glutaraldehyde on the flat end of a platinum disk electrode silanized with 3-aminopropyltrimethoxysilane. Similarly, a phosphate ion enzyme sensor was made by coimmobilizing purine nucleoside phosphorylase and xanthine oxidase on the flat end of a glassy carbon disk electrode. A sulfite sensor was based on the amperometric detection (0.6 V vs. Ag/AgCl) of hydrogen peroxide produced by an enzymatic reaction; the current response was linearly related to the sulfite concentration over the range of 5×10-75×10-4 M. This sensor was highly selective for sulfite, except for a small response to nitrite, thiocyanate and thiosulfate. A phosphate sensor gave a response when inosine (5 mM) was added to a 0.1 M borate buffer solution (pH 7.5) containing phosphate. This sensor was based on the amperometric detection (0.5 V vs. Ag/AgCl) of uric acid produced by two successive enzymatic reactions. The current response was linearly related to the phosphate concentration over a range of 5×10-72×10-5 M at 37±0.2°C. This sensor was essentially specific for phosphate.