Identification of marker genes for intestinal immunomodulating effect of a fructooligosaccharide by DNA microarray analysis
Identification of marker genes for intestinal immunomodulating effect of a fructooligosaccharide by DNA microarray analysis
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DOI：
10.1021/jf062814q
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发表时间：
2007-04-18
影响因子：
6.1
通讯作者：
Kono, Toshiaki
中科院分区：
文献类型：
--
作者：
Fukasawa, Tomoyuki;Murashima, Koichiro;Kono, Toshiaki
益生元低聚果糖因其肠道免疫调节作用而闻名，并且鉴定该作用的标记物是一个非常令人关注的问题。本研究的目的是确定一种特定的低聚果糖（FOS）对宿主动物的生理效应的标记基因，并确定其在小肠中的功能靶点。利用基因芯片技术筛选候选标记基因，并对饲喂低聚果糖后小鼠回肠组织基因表达的变化进行综合研究。FOS的主要生理作用之一是肠道免疫调节。标记基因，然后确定主要组织相容性复合物I类和II类，干扰素，磷脂酰肌醇代谢产物。另外，将回肠分割成派伊尔集合淋巴结（PP）和另一回肠器官（Delta PP），并通过定量RT-PCR方法对它们进行分析，结果识别FOS功能的位点是Delta PP而不是PP。这是第一篇从基因表达水平揭示FOS在小肠生理效应中的标志物的文章。应用这些标记基因将有可能阐明宿主生物体如何识别膳食FOS的施用和肠道环境的相关变化以及其免疫调节作用如何在体内表达的机制。
Prebiotic fructooligosaccharides are noted for their intestinal immunodulating effects, and the identification of markers for the effects is a matter of great concern. This study aimed to identify marker genes for physiological effects of a particular fructooligosaccharide (FOS) on a host animal and also to define the target of its function in the small intestine. DNA microarray technology was used to screen candidate marker genes, and comprehensive changes in gene expressions in the ileum of mice fed with FOS were investigated. One of the major physiological effects of FOS was intestinal immunomodulation. Marker genes were then identified for major histocompatibility complex classes I and II, interferon, and phosphatidylinositol metabolites. Also, the ileum was segmented into Peyer's patch (PP) and the other ileal organ (Delta PP), and these were analyzed by quantitative RT-PCR method, with the result that the site for recognizing the FOS function was the Delta PP rather than the PP. This is the first paper showing the markers for the physiological effects of FOS in the small intestine at gene expression level. Applying these marker genes would make it possible to clarify the mechanisms of how the administration of dietary FOS and associated changes in the intestinal environment are recognized by host organisms as well as how its immunomodulating effects are expressed in the body.