IDENTIFICATION OF EARLY GENES IN THE DROSOPHILA IMMUNE-RESPONSE BY PCR-BASED DIFFERENTIAL DISPLAY - THE ATTACIN-A GENE AND THE EVOLUTION OF ATTACIN-LIKE PROTEINS
IDENTIFICATION OF EARLY GENES IN THE DROSOPHILA IMMUNE-RESPONSE BY PCR-BASED DIFFERENTIAL DISPLAY - THE ATTACIN-A GENE AND THE EVOLUTION OF ATTACIN-LIKE PROTEINS
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DOI：
10.1016/0965-1748(94)00091-c
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发表时间：
1995-04-01
影响因子：
3.8
通讯作者：
HULTMARK, D
中科院分区：
文献类型：
--
作者：
ASLING, B;DUSHAY, MS;HULTMARK, D
我们正在使用基于PCR的差异显示技术来分离果蝇免疫应答过程中诱导的基因。以这种方式，抗菌蛋白的attacin家族的成员的cDNA克隆被分离。相应的Attacin A（AttA）基因位于第二条染色体上的51 A-B，并且它与至少一个交叉杂交基因紧密连锁。注射细菌诱导0.8 kb的转录本，表达动力学类似于天蚕素。果蝇attacin与Sarcophaga peregrina的sarcotoxin II关系最密切，但它缺乏这种蛋白质所特有的额外结构域，并且AttA基因产物的整体结构域结构与Hyalophora cecropia的attacin相同。
We are using the PCR-based differential display technique to isolate genes which are induced during the immune response in Drosophila. In this way, a cDNA clone for a member of the attacin family of antibacterial proteins was isolated. The corresponding Attacin A (AttA) gene is localized at 51A-B on the second chromosome, and it is closely linked to at least one more cross-hybridizing gene. Injection of bacteria induces a 0.8 kb transcript, with expression kinetics similar to that of cecropin. Drosophila attacin is most closely related to sarcotoxin II of Sarcophaga peregrina, but it lacks the extra domains that are unique to this protein, and the overall domain structure of the AttA gene product is identical to that of the attacins from Hyalophora cecropia.